Indian Journal of Science and Technology

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


The study aimed at investigating the genetic diversity of ten different commercial varieties of chilli using random amplified polymorphic DNA (RAPD) analysis. The DNA extracted by CTAB method from the leaf sample was amplified by successive PCR cycles. Using 12 different primers, the amplified DNA solution was subjected to agarose gel electrophoresis. Around 500 different bands were observed under UV light. Seven were polymorphic for a specific primer and can be used as differential markers. This effort can serve determining genetic diversity among ten commercial varieties of chilli in India.

Keywords

Chilli, RAPD, Molecular Markers, RFLP
User