Open Access Open Access  Restricted Access Subscription Access

Antimicrobial, Antidiarrhoeal and Analysis of Phytochemical Constituents of Sphaeranthus Amaranthoides


Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai 600 119, India
2 Department of Bioorganic Chemical Laboratory, Central Leather Research Institute, Chennai 600 020, India
 

The ethnomedicinal value of Spheranthus amaranthoides was investigated through phytochemical study, in vitro antibacterial- and antidiarrhoeal- activity of ethanolic extracts. The analysis of the extracts revealed the presence of flavonoids, carbohydrate, tannins, saponins, steroids, glycosides, terpenoids and alkaloids. The extract evinced antibacterial activity on all the tested organisms viz. Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecalis and Escherichia coli at the concentration ranged from 50- 400µg/ml as assayed by disc diffusion. S. amaranthoides also caused a significant (pSpheranthtus amaranthoides that can be used as a therapeutic agent.

Keywords

Spheranthus amaranthoides, Phytochemicals, Castor Oil Induced Diarrhea
User

  • Arokiyaraj S, Radha R, Martin S and Perinbam K (2008) Phytochemical analysis and anti-diabetic activity of Cadaba fruticosa R.Br. Indian J. Sci.Technol. 1 (6), 1-4. Domain site: http://www.indjst.org
  • Bisignano G, Sanogo R, Marino A, Aquino R, D'Angelo V, Germanò MP, De Pasquale R and Pizza C (2000) Antimicrobial activity of Mitracarpus scaber extract and isolated constituents. Lett. Appl. Microbiol. 30, 105-108
  • Chellaram C and Edward JKP (2009) Anti-inflammatory potential of coral reef associated gastropod, Drupa margariticola. Indian J. Sci.Technol. 2 (2), 75-77. Domain site: http://www.indjst.org.
  • Collins LV, Kristian SA, Weidenmaier C, Faigle M, Van Kessel KP, Van Strijp JA, Götz F, Neumeister B and Peschel A (2002) Staphylococcus aureus strains lacking Dalanine modifications of teichoic acids are highly susceptible to human neutrophil killing and are virulence attenuated in mice. J. Infect. Dis. 186, 214-219.
  • Court WE, Evans WC and Trease GE (1957) The structure of the ischolar_main and stem of Rauwolfia caffra Sond. J. Pharm. Pharmacol. 9, 237-250.
  • Devi BP, Boominathan R and Mandal SC (2002) Evaluation of anti-diarrheal activity of Cleome viscosa L. extract in rats. Phytomedicine. 9, 739-742.
  • Ferreira SH, Herman A and Vane JR (1972) Proceedings: Prostaglandin generation maintains the smooth muscle tone of the rabbit isolated jejunum. Br. J. Pharmacol. 44, 328P-329P.
  • Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocete MA and Jiménez J (1993) Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavonoid constituent. Planta Med. 59, 333-336.
  • Gangadevi V, Yogeswari S, Kamalraj S, Rani G and Muthumary J (2008) The antibacterial activity of Acalypha indica L. Indian J. Sci.Technol. 1 (6), 1-5. Domain site: http://www.indjst.org.
  • Gordon DM (2001) Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. Microbiology. 147, 1079- 1085.
  • Hammer KA, Carson CF and Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts J. Appl. Microbiol. 86, 985-990.
  • Heisig P (2001) Inhibitors of bacterial topoisomerases: mechanisms of action and resistance and clinical aspects. Planta Med. 67, 3-12.
  • Hostettmann K, Pettei MJ, Kubo I and Nakanishi K (1977) Direct obtaining of pure compounds from crude plant extracts by preparative liquid chromatography Lloydia. Helv Chim Acta. 60, 670-672.
  • Ikram M and Innamual H (1980a) Screening of medicinal plants for antimicrobial activity: part 1. Fitoterpia. 51, 231.
  • Ikram M and Innamual H (1980b) Screening of medicinal plants for antimicrobial activity part 2. FH S1:281.
  • Kirtikar KR and Basu BD (1971) Indian Medicinal Plants, Vol.3, 2nd edn. Beshen Singh & Mahandra Pal Singh, DehraDun, pp: 2327-2328.
  • Lorke D (1983) A new approach to practical acute toxicity testing. Arch. Toxicol. 54, 275-287.
  • Mandal SC, Mukherjee PK, Seha K, Pal M and Saha BP (1997) Antidiarrhoeal evaluation of Ficus racemosa Linn. leaf extract. Natural Products Science. 3, 2,100-103.
  • Mc Farland J (1987) Standardization of bacterial Culture for disc diffision assay. J. Am. Medical Assn. 49, 1176-1178.
  • Morebise O, Fafunso MA, Makinde JM, Olajide OA and Awe EO (2002) Antiinflammatory property of the leaves of Gongronema latifolium. Phytother. Res. 16, S75-77.
  • Aiyelagabe.O.O. (2000) Antibacterial activity of Jatropha multifida ischolar_mains. Fitoterapia. 72, 544-546.
  • Mounissamy V, Darimane S and Gunasegaran R (2002) Antibacterial activity of Gosssypetin, isolated from Hibiscus saddriffa the antiseptic. The Antiseptic. 99, 81-82.
  • Morebise O, Fafunso MA, Makinde JM, Olajide OA and Awe EO (2002) Antiinflammatory property of the leaves of Gongronema latifolium. Phytother Res. 16, S75-77.
  • Murray JL, Zukiwski AA, Mujoo K and Rosenblum MG (1990) Recombinant alpha-interferon enhances tumor targeting of an antimelanoma monoclonal antibody in vivo. J. Biol. Response Mod. 9, 556-563.
  • Nostro A, Germanò MP, D'angelo V, Marino A and Cannatelli MA (2000) Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett. Appl. Microbiol. 30, 379-384.
  • Odebiyi OO and Sofowora EA (1978) Phytochemical screening of Nigerian medicinal plants II. Lloydia. 41(3): 234-246.
  • Rath HC, Wilson KH and Sartor RB (1999) Differential induction of colitis and gastritis in HLA-B27 transgenic rats selectively colonized with Bacteroides vulgatus or Escherichia coli. Infect. Immun. 67, 2969-2974.
  • Rehan Ahmad, Swayam Prakash Srivastava, Rakesh Maurya, Rajendran SM, Arya KR and Arvind K. Srivastava (2008) Mild antihyperglycaemic activity in Eclipta alba, Berberis aristata, Betula utilis, Cedrus deodara, Myristica fragrans and Terminalia chebula. Indian J. Sci.Technol. 1 (5), 1-6. Domain site: http://www.indjst.org
  • Sahoo PK, Pillai BR, Mohanty J, Kumari J, Mohanty S and Mishra BK (2007) In vivo humoral and cellular reactions, and fate of injected bacteria Aeromonas hydrophila in freshwater prawn Macrobrachium rosenbergii. Fish Shellfish Immunol. 23, 327-340.
  • Sanders KM (1984) Role of prostaglandins in regulating gastric motility. Am. J. Physiol. 247, G117-126.
  • Tejani A, Pomrantz A, Khawar R and Fusi M (1986) Community acquired infections in children on maintenance cyclosporine therapy. Int. J. Pediatr. Nephrol. 7, 141-144.
  • Trease GE and Evans IC (1983) pharmacognasy (12th edn) Bailliere Tindall London. pp:21-22.
  • Tripathi G (1994) Molecular weight of cytoplasmic malate dehydrogenase, mitochondrial malate dehydrogenase and lactate dehydrogenase of a freshwater catfish. Biomed. Environ. Sci. 7, 122-129.
  • Wink DA, Vodovotz Y, Grisham MB, DeGraff W, Cook JC, Pacelli R, Krishna M and Mitchell JB (1999) Antioxidant effects of nitric oxide. Methods Enzymol. 301, 413-424.
  • Woldemichael GM, Singh MP, Maiesew M and Timmermann BN (2003) Constituents of antibacterial extract of Caesalpinia paraguariensis Burk.2. Nature Forseh. 58c, 70-75.
  • Yu LL, Liao JF and Chen CF (2000) Anti-diarrheal effect of water extract of Evodiae fructus in mice. J. Ethnopharmacol. 73, 39-45.
  • Zavala I, Barrera E and Nava A (1988) Ceftriaxone in the treatment of bacterial meningitis in adults. Chemotherapy. 34, 47-52.

Abstract Views: 476

PDF Views: 236




  • Antimicrobial, Antidiarrhoeal and Analysis of Phytochemical Constituents of Sphaeranthus Amaranthoides

Abstract Views: 476  |  PDF Views: 236

Authors

L. Swarna Latha
Department of Biotechnology, Sathyabama University, Chennai 600 119, India
P. Neelakanta Reddy
Department of Bioorganic Chemical Laboratory, Central Leather Research Institute, Chennai 600 020, India

Abstract


The ethnomedicinal value of Spheranthus amaranthoides was investigated through phytochemical study, in vitro antibacterial- and antidiarrhoeal- activity of ethanolic extracts. The analysis of the extracts revealed the presence of flavonoids, carbohydrate, tannins, saponins, steroids, glycosides, terpenoids and alkaloids. The extract evinced antibacterial activity on all the tested organisms viz. Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecalis and Escherichia coli at the concentration ranged from 50- 400µg/ml as assayed by disc diffusion. S. amaranthoides also caused a significant (pSpheranthtus amaranthoides that can be used as a therapeutic agent.

Keywords


Spheranthus amaranthoides, Phytochemicals, Castor Oil Induced Diarrhea

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i3%2F29413