Indian Journal of Science and Technology

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Establishment of Mature Axillary Bud Culture of Sugarcane and overcoming Persistent Culture Contamination


Affiliations
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, Canada
2 Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, China
 

Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.

Keywords

Axillary Bud Culture, Tissue Culture Contamination, Disease Free Stock, Sugarcane
User

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  • Establishment of Mature Axillary Bud Culture of Sugarcane and overcoming Persistent Culture Contamination

Abstract Views: 434  |  PDF Views: 246

Authors

M. E. Wagiha
Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, Canada
S. W. Adkinsb
Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
K. Attia
Institute of Genetic Engineering, Fudan University, Shanghai, 200433, China

Abstract


Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.

Keywords


Axillary Bud Culture, Tissue Culture Contamination, Disease Free Stock, Sugarcane

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i1%2F29365