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Seaweed Extracts Control the Leaf Spot Disease of the Medicinal Plant Gymnema Sylvestre


Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, India
 

Antimicrobial screening of 12 different seaweeds extracts namely Chaetomorpha antennina, Dictyota dichotoma, Enteromorpha flexuosa, Laurencia obtusa, Gracilaria corticata, Gracilaria verrucosa, Grateloupia lithophila, Padina boergesenii, Sargassum wightii, Turbinaria conoides, Halimeda tuna and Ulva lactuca was carried out in vitro. The crude extracts were tested against the phytopathogenic bacterium- Pseudomonas syringae causing leaf spot disease of the medicinal plant Gymnema sylvestre. The methanolic extracts of Sargassum wightii showed maximum activity followed by ethyl acetate compared to that of other organic solvent- extracts. Thus, this investigation throws fresh light on the appropriate usage of solvent extraction method in preparing potent biopesticide.

Keywords

Gymnema Sylvestre, Seaweed Extracts, Plant Pathogenic Bacteria, Leaf Spot Disease, Pathogenicity, Pseudomonas Syringae
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  • Arun Kumar K and Regnasamy R (2000) Antibacterial activities of seaweed extracts/fractions obtained through a TLC profile against phytopathogenic bacterium Xanthomonas oryzae sp oryzae. Bot. Mar. 43, 417-421.
  • Arun Kumar K and Regnasamy R (2000) Evaluation of Antibacterial potential of seaweeds occurring along the coast of Tamilnadu, India against the plant pathogenic bacterium Xanthomonas oryzae Pv. oryzae (Ishiyama) Dye. Bot. Mar. 43, 409-415.
  • Arun Kumar K, Selvapalam N and Regnasamy R (2005) The antibacterial compound Sulphoglycerolipid 1-0 palmitoyl-3-0 (6'-sulpho- a-quinovopyranosyl)-glycerol from Sargassum wightii. Bot. Mar. 48, 441-445.
  • Bauer AW, Kirby WM, Sherris JC and Turk M (1966) Antibiotic susceptibility by a standardized single disk method. Am. J. Clin. Pathol. 45: 493- 496.
  • Boucher C, Genin S and Arlat M (2001) Current concepts of pathogenicity in plant pathogenic bacteria. Life Sci. 324, 915-922.
  • Caccamese S and Azzolina R (1979) Screening for antimicrobial activities in marine algae from Eastern Sicily. Planta Med. 37, 333-339.
  • Caccamese S, Azzolina R, Furnari G, Cormaci M and Grasso S (1980) Antimicrobial and antiviral activities of extracts from Mediterranean algae. Bot. Mar. 23, 285-288.
  • Darcy-Vrillon B (1993) Nutritional aspects of the developing use of marine macroalgae for the human food industry. Int. J. Food Sci. Nutr. 44, S23-S35.
  • Devienne KF and Raddi MSG (2002) Screening for antimicrobial activity of natural products using a microplate photometer. Braz. J. Microbiol. 33, 166-168.
  • Goto M (1992) Fundamentals of bacterial plant pathology. Pages 210-224 In: Academic Press, San Diego, CA.
  • Hindler JA, González AH and Drake TA (1990) Stability of viable bacterium counts in liquid media used for preparation of inocula and subsequent impact on antimicrobial susceptibility test results. J. Clinical Microbiol. 28, 1271-1275.
  • Holt JG, Krieg NR, Sneath PHA and Staley JT (1994) Bergey’s manual of determinative bacteriology. Ninth edition, Williams and Willkins Company, Ballamore, MD, USA, pp.255-273.
  • Indergaard M and Minsaas J (1991) Animal and human nutrition. In: Guiry, M.D., Blunden, G. (Eds.), Seaweed Resources in Europe: Uses and Potential. Wiley, Chichester, pp. 21- 64.
  • Klement Z (1963) Rapid detection of the pathogenicity of phytopathogenic Pseudomonas. Nature (London) 199, 299- 300.
  • Koch R (1881) Zur untersuchung von pathogenen organismen. In: Mitth A, Kaiserl D (Eds) Gesundheitsampte 1: 1-48 Milestones in Microbiology 1556 to 1940, translated and edited by Thomas D. Brock, ASM Press. 1998, p.101.
  • Kulik MM (1995) The potential for using Cyanobacteria (blue-green algae) and algae in the biological control of plant pathogenicbacteria and fungi. Eur. J. Pl. Pathol. 101, 35-599
  • Lewis JG, Stanley NF and Guist GG (1988) In: Lembi CA, Waaland JR (Eds.), Algae and Human A.airs. Cambridge University Press, New York, pp. 205-236.
  • Lourdu Mariadoss K and Rengasamy R (1995) Studies on seaweed extracts against phytopathogenic bacterium, Xanthomonas oryzae pv. Oryzae. Mar. Res. 4, 121-126.
  • Lourdu Mariadoss K (1998) Studies on extraction, isolation and characterization of bioactive compounds from seaweeds and their effect on bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae (Ishiyama) dye. Ph.D. Thesis. University of Madras, Chennai, India.
  • Manimala K and Rengasamy R (1993) Effect of bioactive compounds of seaweeds on the phytopathogen Xanthomonas oryzae. Phycos. 32, 77-83.
  • Mazumder S (2006) Industrial polysaccharides from natural sources: structure and function. Ph.D. Thesis, The University of Burdwan, Burdwan, India. pp. 1-69.
  • Mazzanti G, Mascellino MT, Battinelli L, Coluccia D, Manganaro M and Saso L (2000) Antimicrobial investigation of semipurified fractions of Ginkgo biloba leaves. J. Ethnopharmacol. 71, 83-88.
  • National Committee for Clinical laboratory Standards (2000) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically.5th Edn. Approved Standard M7-A5.
  • Pesando D and Caram B (1984) Screening of marine algae from the French Mediterranean Coast for antibacterial and antifungal activity. Bot. Mar. 27, 381-386.
  • Rao PPS, and Parekh KS 1981. Antibacterial activity of Indian seaweed extracts. Bot. Mar. 24, 577-582.
  • Rao PPS (1991) Biological investigation of Indian marine algae, screening of some green, red and brown seaweeds for their antimicrobial activity. Seaweed Res. Util. 14, 37-43.
  • Reichelt JL and Borowitzka MA (1984) Antimicrobial activity from marine algae: results of a large scale screening program. Hydrobiologia 116/117, 158-167.
  • Renn D (1997) Biotechnology and the red seaweed polysaccharide industry: status, needs and prospects. Tibtech 15, 9-14.
  • Skjak-Braekb G, Martinsen A (1991) In: Guiry MD, Blunden G (Eds.), Seaweed Resources in Europe: Uses and Potential. John Wiley & Sons, pp. 219-256.
  • Sonnenwirth CA, Jarett L (1980) Gradwohl’s Clinical Laboratory Methods and Diagnosis, 8th Edn. C.V. Mosby Company, St. Louis. pp.1959-1970.
  • Stephen AM (1995) Food Polysaccharides and Their Applications. Marcel Dekker, New York.
  • Stiger V, Deslandes E, Elisabeth and Payri C (2004) Phenolic contents of two brown algae, Turbinaria ornata and Sargassum mangarevense on Tahiti (French Polynesia): interspecific, ontogenic and spatio-temporal variations. Bot. Mar. 47, 402-409.
  • Sultana VS, Haque E, Ara J and Athar M (2005) Comparative efficacy of brown, green and red seaweeds in the control of ischolar_main infecting fungi and okra. Int. J. Environ. Sci. Tech. 2, 129-132
  • Zilinskas RA and Lundin CG (1993) Marine Biotechnology and Developing Countries (World Bank Discussion Paper, No. 210). The World Bank. pp. 29.

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  • Seaweed Extracts Control the Leaf Spot Disease of the Medicinal Plant Gymnema Sylvestre

Abstract Views: 753  |  PDF Views: 150

Authors

Chinnadurai Sreenath Kumar
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, India
Dronamraju V. L. Sarada
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, India
Ramasamy Rengasamy
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai - 600 025, India

Abstract


Antimicrobial screening of 12 different seaweeds extracts namely Chaetomorpha antennina, Dictyota dichotoma, Enteromorpha flexuosa, Laurencia obtusa, Gracilaria corticata, Gracilaria verrucosa, Grateloupia lithophila, Padina boergesenii, Sargassum wightii, Turbinaria conoides, Halimeda tuna and Ulva lactuca was carried out in vitro. The crude extracts were tested against the phytopathogenic bacterium- Pseudomonas syringae causing leaf spot disease of the medicinal plant Gymnema sylvestre. The methanolic extracts of Sargassum wightii showed maximum activity followed by ethyl acetate compared to that of other organic solvent- extracts. Thus, this investigation throws fresh light on the appropriate usage of solvent extraction method in preparing potent biopesticide.

Keywords


Gymnema Sylvestre, Seaweed Extracts, Plant Pathogenic Bacteria, Leaf Spot Disease, Pathogenicity, Pseudomonas Syringae

References





DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i3%2F29225