Indian Journal of Science and Technology

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Sub-cellular Localization of Suppressor of Potassium Transport Defect 3 (SKD3) into Nucleus and Nuclear Matrix


Affiliations
1 Centre for Cellular and Molecular Biology, Hyderabad, India
2 National Institute of Nutrition, Hyderabad, India
3 Jawaharlal Nehru Technological University, Hyderabad, India
 

Suppressor of Potassium Transport Defect 3 (SKD3) is a mammalian homologue of Escherichia coli ClpB and S. cerevisiae Hsp104. While the exact cellular functions of SKD3 are not known, the functions of its microbial and yeast homologues have been well documented. The study presents new evidence on the localization of the protein under stress conditions like heat shock and H2O2 treatment. Immunofluorescence study of heat stressed cells showed that the protein was redistributed to large foci in the nucleus while cytoplasmic staining remained unaltered. Upon H2O2 treatment, the SKD3 protein level is increased both in the cytoplasm and nucleus. The outcome of this study reveals the fact that SKD3 may be a component of nucleus as well as nuclear scaffold which has a direct regulatory role during stress.

Keywords

SKD3, ClpB, HSP104, Nuclear Matrix, Oxidative Stress, Heat Shock
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  • Sub-cellular Localization of Suppressor of Potassium Transport Defect 3 (SKD3) into Nucleus and Nuclear Matrix

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Authors

S. Thanumalayan
Centre for Cellular and Molecular Biology, Hyderabad, India
V. Venkatesan
National Institute of Nutrition, Hyderabad, India
M. Laxmi Narasu
Jawaharlal Nehru Technological University, Hyderabad, India

Abstract


Suppressor of Potassium Transport Defect 3 (SKD3) is a mammalian homologue of Escherichia coli ClpB and S. cerevisiae Hsp104. While the exact cellular functions of SKD3 are not known, the functions of its microbial and yeast homologues have been well documented. The study presents new evidence on the localization of the protein under stress conditions like heat shock and H2O2 treatment. Immunofluorescence study of heat stressed cells showed that the protein was redistributed to large foci in the nucleus while cytoplasmic staining remained unaltered. Upon H2O2 treatment, the SKD3 protein level is increased both in the cytoplasm and nucleus. The outcome of this study reveals the fact that SKD3 may be a component of nucleus as well as nuclear scaffold which has a direct regulatory role during stress.

Keywords


SKD3, ClpB, HSP104, Nuclear Matrix, Oxidative Stress, Heat Shock

References





DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i3%2F29220