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Differential Expression of PML in 60Co γ-ray and γ-IFN- Induced Apoptosis in B-lymphocytes


Affiliations
1 Department of Hematology, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu 226001, China
2 Department of Radiobiology, Institute for Environmental Sciences, Hachazawa 2-121, Takahoko, Rokkasho, Kamikita, Aomori 139-3212, Japan
3 Hiroshima Atomic Bomb Relief Foundation, Kurakake, Asakita-ku, Hiroshima 739-1743, Japan
 

The function of PML in apoptosis was elucidated in human B lymphocyte- derived cell line. For this purpose, the number of the PML focus and the percentage of apoptotic cells were determined periodically with immunofluorescent staining after 60Co γ-ray or γ-interferons (γ-IFN) treatment. On irradiation with 60Co γ-ray or treatment with γ-IFN, PML protein was over-expressed. This was measured from PML foci, which peaked at 72 hr and 24 hr, respectively. The B-cell line also contained a greater proportion of apoptotic cells after the treatments. The strongest induction of apoptosis both by 60Co- γray irradiation and by γ-IFN treatment was observed 24 hr later than the induction of PML expression. In addition, γ- rays-induced apoptosis and PML expression were mediated through caspase-8 but not through caspase-3. However, caspase-8 was involved in γ-IFN-induced PML expression and apoptosis. While caspase-3 is involved solely in PML expression, and partially in apoptosis. These results suggest that 60Co- γ ray irradiation or γ-IFN treatment can induce PML protein expression and apoptosis in the B-cell line as caspase-3 dependently or independently.

Keywords

PML Protein, Apoptosis, 60Co-γ Ray, γ-IFN, Caspase Activation
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  • Differential Expression of PML in 60Co γ-ray and γ-IFN- Induced Apoptosis in B-lymphocytes

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Authors

Hong Liu
Department of Hematology, Affiliated Hospital of Nantong Medical College, Nantong, Jiangsu 226001, China
Kimio Tanaka
Department of Radiobiology, Institute for Environmental Sciences, Hachazawa 2-121, Takahoko, Rokkasho, Kamikita, Aomori 139-3212, Japan
Nanao Kamada
Hiroshima Atomic Bomb Relief Foundation, Kurakake, Asakita-ku, Hiroshima 739-1743, Japan

Abstract


The function of PML in apoptosis was elucidated in human B lymphocyte- derived cell line. For this purpose, the number of the PML focus and the percentage of apoptotic cells were determined periodically with immunofluorescent staining after 60Co γ-ray or γ-interferons (γ-IFN) treatment. On irradiation with 60Co γ-ray or treatment with γ-IFN, PML protein was over-expressed. This was measured from PML foci, which peaked at 72 hr and 24 hr, respectively. The B-cell line also contained a greater proportion of apoptotic cells after the treatments. The strongest induction of apoptosis both by 60Co- γray irradiation and by γ-IFN treatment was observed 24 hr later than the induction of PML expression. In addition, γ- rays-induced apoptosis and PML expression were mediated through caspase-8 but not through caspase-3. However, caspase-8 was involved in γ-IFN-induced PML expression and apoptosis. While caspase-3 is involved solely in PML expression, and partially in apoptosis. These results suggest that 60Co- γ ray irradiation or γ-IFN treatment can induce PML protein expression and apoptosis in the B-cell line as caspase-3 dependently or independently.

Keywords


PML Protein, Apoptosis, 60Co-γ Ray, γ-IFN, Caspase Activation

References





DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i3%2F29219