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Removal and Recovery of Mercury in Vitro Using Immobilized Live Biomass of Chlorella sp.


Affiliations
1 Agricultural Bioprospection Research Group, University of Sucre, Sincelejo, Colombia
 

Objectives: This research was aimed at evaluating the capacity of Mercury removal and recovery using live biomass of Chlorella sp. immobilized in scourer (Luffa cylindrica). Methods/Statistical Analysis: The algal biomass was bioaugmented in photobioreactors with 4 mM Agrimins during 20 days in constant agitation. For the immobilization of the microalga, scourer fragments were used. The mercury removal and/or desorption capacity was determined during 24 h, and desorption was carried out by acid digestion. An ANOVA and the Tukey test for significant differences (p-value ≤ 0.05) were performed with the results in the InfoStat software. Findings: an average immobilization of Chlorella sp. of 1.69g of algal cells/scourer fragment after 20 days of incubation. The statistical analysis showed significant statistical differences between all removal times, presenting the highest averages at 24 h of exposure, with a Mercury removal of 98.58% and a desorption of 82.61%. Likewise, in the lowest concentrations the microalga showed greater capacity of Mercury sorption, while at the highest concentrations the desorption of said heavy metal was greater. Improvements/Applications: Chlorella in bioremediation techniques of heavy metals are positioned as a biotechnological alternative, which thanks to its high rate of removal and desorption allows the ecological disposition of the contaminant.
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  • Zeraatkar AK, Ahmadzadeh H, Talebi AF, Moheimani NR, McHenry MP. Potential use of algae for heavy metal bioremediation, a critical review, Journal of Environmental Management. 2016; 181:817-31. https://doi.org/10.1016/j.jenvman.2016.06.059. PMid: 27397844.

  • Malar S, Sahi SV, Favas PJC, Venkatachalam P. Mercury heavy-metal-induced physiochemical changes and geno toxic alterations in water hyacinths [Eichhornia crassipes (Mart.)], Environment Science and Pollution Research. 2015; 22(6):4597-608. https://doi.org/10.1007/s11356-0143576-2. PMid: 25323404.

  • Kumari S, Chauhan GS. New cellulose-lysine Schiff-basebased sensor-adsorbent for mercury ions, ACS Applied Materials and Interfaces. 2014; 6:5908-917. https://doi.org/10.1021/am500820n. PMid: 24654907.

  • Carolin CF, Kumar PS, Saravanan A, Joshiba GJ, Naushad M. Efficient techniques for the removal of toxic heavy metals from aquatic environment: A review, Journal of Environmental Chemical Engineering. 2017; 5(3):2782-99. https://doi.org/10.1016/j.jece.2017.05.029.

  • Hadavifar M, Bahramifar N, Younesi H, Rastakhiz M, Li Q, Yu J, Eftekhari E. Removal of mercury(II) and cadmium(II) ions from synthetic wastewater by a newly synthesized amino and thiolated multi-walled carbon nanotubes, Journal of the Taiwan Institute of Chemical Engineers. 2016; 67:397-405. https://doi.org/10.1016/j.jtice.2016.08.029.

  • Gao S, Yang C, Tang L, Zhu J, Xu Y, Wang S, Chen F. Growth and antioxidant responses in Jatropha curcas seedling exposed to mercury toxicity, Journal of Hazard Mater. 2010; 182(1-3):591-97. https://doi.org/10.1016/j.jhazmat.2010.06.073. PMid: 20655143.

  • Gill SS, Khan N, Tuteja, N. Cadmium at high dose perturbs growth, photosynthesis and nitrogen metabolism while at low dose it up regulates sulphur assimilation and antioxidant machinery in garden cress (Lepidium sativum L.), Plant Science. 2012; 182:112-20. https://doi.org/10.1016/j.plantsci.2011.04.018. PMid: 22118622.

  • Crowe W, Allsopp PJ, Watson GE, Magee PJ, Strain JJ, Armstrong DJ, Ball E, McSorley EM. Mercury as an environmental stimulus in the development of autoimmunityA systematic review, Autoimmunity Reviews. 2017; 16(1): 72-80. https://doi.org/10.1016/j.autrev.2016.09.020. PMid: 27666813.

  • Lee SM, Laldawngliana C, Tiwari D. Iron oxide nanoparticlesimmobilized-sand material in the treatment of Cu(II), Cd(II) and Pb(II) contaminated wastewaters, Chemical Engineering Law. 2012; 195-196:103-11. https://doi.org/10.1016/j.cej.2012.04.075.

  • Goharshadi EK, Moghaddam MB. Adsorption of hexavalent chromium ions from aqueous solution by graphene nanosheets: kinetic and thermodynamic studies, International Journal of Environmental Science and Technology. 2015; 12(7):2153-60.

  • Kuppusamy S, Thavamani P, Venkateswarlu K, Lee K, Naidu R, Megharaj M. Remediation approaches for Polycyclic Aromatic Hydrocarbons (PAHs) contaminated soils: Technological constraints, emerging trends and future directions, Chemosphere. 2017; 168:944-68. https://doi.org/10.1016/j.chemosphere.2016.10.115. PMid: 27823779.

  • Jacob JM, Karthik C, Saratale RG, Kumar SS, Prabakar D, Kadirvelu K, Pugazhendhi A. Biological approaches to tackle heavy metal pollution: A survey of literature, Journal of Environmental Management. 2018; 217:56-70. https://doi.org/10.1016/j.jenvman.2018.03.077. PMid: 29597108.

  • Favara P, Gamlin J. Utilization of waste materials, nonrefined materials, and renewable energy in situ remediation and their sustainability benefits, Journal of Environmental Management. 2017; 204:730-37. https://doi.org/10.1016/j.jenvman.2017.03.097. PMid: 28390816.

  • Vasilieva S, Shibzukhova K, Morozov A, Solovchenko A, Bessonov I, Kopitsyna M, Lukyanov A, Chekanov K, Lobakova E. Immobilization of microalgae on the surface of new cross-linked polyethylenimine-based sorbents, Journal of Biotechnology. 2018; 281:31-38. https://doi.org/10.1016/j.jbiotec.2018.03.011. PMid: 29654799.

  • de-Bashan LE, Bashan Y. Immobilized microalgae for removing pollutants: Review of practical aspects, Bioresource Technology. 2010; 101(6):1611-27. https://doi.org/10.1016/j.biortech.2009.09.043. PMid: 19931451.

  • Kumar KS, Dahms HU, Won EJ, Lee JS, Shin KH. Microalgae-A promising tool for heavy metal remediation, Ecotoxicology and Environmental Safety. 2015; 113: 329-52. https://doi.org/10.1016/j.ecoenv.2014.12.019. PMid: 25528489.

  • Figueira P, Henriques B, Teixeira A, Lopes CB, Reis AT, Monteiro RJ, Duarte AC, Pardal MA, Pereira E. Comparative study on metal biosorption by two macroalgae in saline waters: Single and ternary systems, Environmental Science and Pollution Research. 2016; 23(12):11985-97. https://doi.org/10.1007/s11356-016-6398-6. PMid: 26961530.

  • Henriques B, Lopes CB, Figueira P, Rocha LS, Duarte AC, Vale C, Pardal MA, Pereira E. Bioaccumulation of Hg, Cd and Pb by Fucus vesiculosus in single and multi-metal contamination scenarios and its effect on growth rate, Chemosphere. 2017; 171:208-22. https://doi.org/10.1016/j.chemosphere.2016.12.086. PMid: 28024206.

  • Michalak I, Chojnacka K. Interactions of metal cations with anionic groups on the cell wall of the macroalga Vaucheria sp., Engineering in Life Sciences. 2010; 10(3):209-17. https://doi.org/10.1002/elsc.200900039.

  • Moreno-Garrido I. Microalgae immobilization: Current techniques and uses, Bioresource Technology. 2008; 99(10): 3949-64. https://doi.org/10.1016/j.biortech.2007.05.040. PMid: 17616459.

  • Infante C, Angulo E, Zárate A, Florez JZ, Barrios F, Zapata C. Propagación de la microalga Chlorella sp. en cultivo por lote: Cinética del crecimiento celular, Avances en Ciencias e Ingeniería. 2012; 3(2):159-64.

  • Sánchez E, Garza M, Almaguer V, Sáenz I, Li-án A. Estudio cinético e isotermas de adsorción de Ni (II) y Zn (II) utili zando biomasa del alga Chlorella sp. Inmovilizada, Ciencia Universidad Nacional de Colombia. 2008;11(2):168-76.

  • Nabizadeh R, Naddafi K, Mesdaghinia A, Nafez AH. Feasibility study of organic matter and ammonium removal using loofa sponge as a supporting medium in an Aerated Submerged Fixed-Film Reactor (ASFFR), Electron Journal of Biotechnology. 2008; 11(4):1-9. https://doi.org/10.2225/vol11-issue4-fulltext-8.

  • Akhtar N, Iqbal J, Iqbal M. Removal and recovery of nickel (II) from aqueous solution by loofa sponge-immobilized biomass of Chlorella sorokiniana: Characterization studies, Journal of Hazardous Materials. 2004; 108(1-2):85-94. https://doi.org/10.1016/j.jhazmat.2004.01.002. PMid: 15081166.

  • Ahmad A, Bhat AH, Buang A. Biosorption of transition metals by freely suspended and Ca-alginate immobilised with Chlorellavulgaris: Kinetic and equilibrium modeling, Journal of Cleaner Production. 2018; 171:1361-75. https://doi.org/10.1016/j.jclepro.2017.09.252.

  • Hoang NPV, Xuan TB, Thanh TN, Dinh DN, Dao TS, Cao NDT, Vo TKQ. Effects of nutrient ratios and carbon dioxide bio-sequestration on biomass growth of Chlorella sp. in bubble column photobioreactor, Journal of Environmental Management. 2018; 219:1-8. https://doi.org/10.1016/j.jenvman.2018.04.109. PMid: 29715637.

  • Wiesberg IL, Brigagão GV, de Medeiros JL, de Queiroz Fernandes Araújo O. Carbon dioxide utilization in a microalgabased biorefinery: efficiency of carbon removal and economic performance under carbon taxation, Journal of Environmental Management. 2017; 203:988-98. https://doi.org/10.1016/j.jenvman.2017.03.005. PMid: 28284810.

  • Liu YK, Seki M, Tanaka H, Furusaki S. Characteristics of loofa (Luffa cylindrica) sponge as a carrier for plant cell immobilization, Journal of Fermentation and Bioengineering. 1998; 85:416-21. https://doi.org/10.1016/ S0922-338X(98)80086-X.

  • Kumar SD, Santhanam P, Jayalakshmi T, Nandakumar R, Ananth S, Devi AS, Balaji Prasath B. Optimization of pH and retention time on the removal of nutrients and heavy metal (zinc) using immobilized marine microalga Chlorella marina, Journal of Biological Sciences. 2013; 13:400-05. https://doi.org/10.3923/jbs.2013.400.405.

  • Adam S, Kumar PS, Santhanam P, Kumar SD, Prabhavathi P. Bioremediation of tannery wastewater using immobilized marine microalga Tetraselmis sp.: Experimental studies and pseudo-second order kinetics, Journal of Marine Biology and Oceanography. 2015; 4(1):1-11.

  • Kumar SD, Santhanam P, Park MS, Kim MK. Development and application of a novel immobilized marine microalgae biofilter system for the treatment of shrimp culture effluent, Journal of Water Process Engineering. 2016; 13:137-42. https://doi.org/10.1016/j.jwpe.2016.08.014.

  • Hameed MA. Effect of algal density in bead, bead size and bead concentrations on wastewater nutrient removal, African Journal of Biotechnology. 2007; 6(10):1185-91.

  • Perales-Vela HV, Pe-a-Castro JM, Ca-izares-Villanueva RO. Heavy metal detoxification in eukaryotic microalgae, Chemosphere. 2006; 64(1):1-10. https://doi.org/10.1016/j.chemosphere.2005.11.024. PMid: 16405948.

  • Cobbett C, Goldsbrough P. Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis, Annual Review of Plant Biology. 2002; 53:159-82. https://doi.org/10.1146/annurev.arplant.53.100301.135154. PMid: 12221971.

  • Godlewska-Żyłkiewicz B. Analytical applications of living organisms for preconcentration of trace metals and their speciation, Critical Reviews in Analytical Chemistry. 2001; 31(3):175-89. https://doi.org/10.1080/20014091076730.

  • Volesky B. Biosorption and me, Water Research. 2007; 41(18):4017-29. https://doi.org/10.1016/j.watres.2007.05.062. PMid: 17632204.

  • Arief VO, Trilestari K, Sunarso J, Indraswati N, Ismadji S. Recent progress on biosorption of heavy metals from liquids using low cost biosorbents: Characterization, biosorption parameters and mechanism studies, Clean. 2008; 36(12):937-62. https://doi.org/10.1002/clen.200800167.

  • Kaplan D. Absorption and adsorption of heavy metals by microalgae. Richmond and Hu (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Blackwell Publishing, Israel; 2013. p. 439-47. https://doi.org/10.1002/9781118567166.ch32.

  • Wang J, Chen C. Biosorption of heavy metals by Saccharomyces cerevisiae: A review Biotechnol, Advances. 2006; 24(5):427-51. https://doi.org/10.1016/j.biotechadv. 2006.03.001. PMid: 16737792.

  • Perpetuo EA, Souza C, Oller C. Engineering Bacteria for Bioremediation, Progress in Molecular and Environmental Bioengineering-from Analysis and Modeling to Technology Applications. Carpi (Ed.). Rijeka, Croatia; 2011. p. 646.

  • Kong QX, Li L, Martinez B, Chen P, Ruan R. Culture of microalgae Chlamydomonas reinhardtii in wastewater for biomass feedstock production, Applied Biochemistry and Biotechnology. 2010; 160(1):9-18. https://doi.org/10.1007/ s12010-009-8670-4. PMid: 19507059.

  • Fu F, Wang Q. Removal of heavy metal ions from wastewaters: A review, Journal of Environmental Management. 2011; 92(3):407-18 https://doi.org/10.1016/j.jenvman.2010.11.011. PMid: 21138785.


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  • Removal and Recovery of Mercury in Vitro Using Immobilized Live Biomass of Chlorella sp.

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Authors

Sandra Benítez Alvis
Agricultural Bioprospection Research Group, University of Sucre, Sincelejo, Colombia
Alexander Perez Cordero
Agricultural Bioprospection Research Group, University of Sucre, Sincelejo, Colombia
Deimer Vitola Romero
Agricultural Bioprospection Research Group, University of Sucre, Sincelejo, Colombia

Abstract


Objectives: This research was aimed at evaluating the capacity of Mercury removal and recovery using live biomass of Chlorella sp. immobilized in scourer (Luffa cylindrica). Methods/Statistical Analysis: The algal biomass was bioaugmented in photobioreactors with 4 mM Agrimins during 20 days in constant agitation. For the immobilization of the microalga, scourer fragments were used. The mercury removal and/or desorption capacity was determined during 24 h, and desorption was carried out by acid digestion. An ANOVA and the Tukey test for significant differences (p-value ≤ 0.05) were performed with the results in the InfoStat software. Findings: an average immobilization of Chlorella sp. of 1.69g of algal cells/scourer fragment after 20 days of incubation. The statistical analysis showed significant statistical differences between all removal times, presenting the highest averages at 24 h of exposure, with a Mercury removal of 98.58% and a desorption of 82.61%. Likewise, in the lowest concentrations the microalga showed greater capacity of Mercury sorption, while at the highest concentrations the desorption of said heavy metal was greater. Improvements/Applications: Chlorella in bioremediation techniques of heavy metals are positioned as a biotechnological alternative, which thanks to its high rate of removal and desorption allows the ecological disposition of the contaminant.

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DOI: https://doi.org/10.17485/ijst%2F2018%2Fv11i45%2F137575