Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Lead Resistance by Bacillus cereus 1DH1LIM Isolated from Contaminated Environments with Mercury


Affiliations
1 Sucre University, Agricultural Sciences, Agricultural Bioprospecting Group. Campus Universitario Puerta Roja, AA 406, Sincelejo - Sucre, Colombia
2 Sucre University, Health Sciences, Campus Universitario Puerta Blanca, AA 406, Sincelejo - Sucre, Colombia
3 University of Sucre, Education and Sciences, Bioprospecting Agricultural Group. Campus Universitario Puerta Roja, AA 406, Sincelejo, Sucre, Colombia
 

Objective: Evaluated resistance capacity in vitro of Bacillus cereus 1DH1LIM at different concentrations of lead in the form of Pb (NO3)2. Materials and Statistical Analysis: B. cereus was purified, aliquots of suspensions in log phase were inoculated into minimal medium tris-MMT with different concentrations of lead in the form of Pb (NO3)2 and incubated by stirring at 150 rpm at 32 °C for 120 hours; growth was determined by turbidimetry at 600 nm every hour for four days. Siderophore production was determined by growth on médium azurol-S (CAS). Findings: B. cereus 1DH1LIMIt shows statistical difference with respect to adaptation time and concentration of lead metal present in the medium. At concentrations of 100, 150 and 200 ppm of Pb an adaptation phase of 4 hours, with respect to concentrations of 250 to 400 ppm which lasted 7 hours was observed. The highest growth of B. cereus 1DH1LIMIt was observed at 100 ppm, 150 ppm and 200 ppm and less at400. In CAS medium the bacterial culture exhibited siderophore production. Applications: The findings of this study expand the knowledge to use this endophytic bacteria asa biological resource to remedy lead-contaminated environments.
User

  • Yu H, Ni S J, He Z W, Zhang C J, Nan X, Kong B, Weng ZY. Analysis of the spatial relationship between heavy metals in soil and human activities based on landscape geochemical interpretation, Journal of Geochemical Exploration. 2014 Novl; 146:136–48. https://doi.org/10.1016/j.gex-plo. 2014.08.010.

  • Kossoff D, Dubbin WE, Alfredsson M, Edwards SJ, Macklin MG, Hudson-Edwards K A. Mine tailings dams: Characteristics, failure, environmental impacts, and remediation, Applied Geochemistry. 2014 Dec; 51:229–45. https://doi.org/10.1016/j.apgeochem.2014.09.010.

  • Cheng SF, Huang CY, Lin YC, Lin SC, Chen KL. Phytoremediation of lead using corn in contaminated agricultural land- an in situ study and benefit assessment, Ecotoxicology and Environmental Safety. 2015 Jan; 111:72–77. https://doi.org/10.1016/j.ecoenv.2014.09.024 PMid: 25450917.

  • Dauvin JC. Effects of heavy metal contamination onthe macrobenthic fauna in estuaries:the case of the Seinee stuary, Marine Pollution Bulletin. 2008 Nov; 57(1-5):160–67. https://doi.org/10.1016/j.marpolbul.2007.10.012. PMid: 18045624.

  • Flora SJS, Mittal M, Mehta A. Heavy metal induced oxidative stress and its possible reversal by chelation therapy, Indian Journal of Medical Research. 2008 Oct; 128:501–23. PMid: 19106443.

  • Lombardi PE, Peri SI, Verrengia NR. ALA-D and ALA-D reactivated as biomarkers of lead contamination in the fish Prochilodus lineatus, Ecotoxicology and Environmental Safety. 2010 Oct; 73(7):1704–11. https://doi.org/10.1016/j.ecoenv.2010.06.005. PMid: 20599271.

  • Hartwig A, Asmuss M, Ehleben I, Herzer U, Kostelac D, Pelzer A., Schwerdtle T, Burkle A. Interference by toxic metal ions with DNA repair processes and Cell cycle control: Molecular mechanisms, Environmental Health. Perspectives. 2002 Oct; 110:797–99. https://doi.org/10.1289/ehp.02110s5797. PMid: 12426134, PMCid:PMC1241248.

  • Ahluwalia SS, Goyal D. Microbial and plant derived biomass for removal of heavy metals from wastewater, Bioresource Technology. 2008 Sep; 98(12):2243–57. https://doi.org/10.1016/j.biortech.2005.12.006. PMid: 16427277.

  • Naik MM, Pandey A, Dubey SK. Pseudomonas aeruginosa strain WI-1 from Mandovi estuary possesses metallothione in to alleviate lead toxicity and promotes plant growth, Ecotoxicology and Environmental Safety. 2012 May; 79:129–33. https://doi.org/10.1016/j.ecoenv.2011.12.015. PMid: 22284824.

  • Naik MM, Pande A, Dubey SK. Biological characterization of lead- enhanced exopolysaccharide produced by a lead resistant Enterobacter cloacae strain P2B, Biodegradation. 2012 Sep; 23(5):775–83. https://doi.org/10.1007/s10532-012-9552-y. PMid: 22544353.

  • Naik MM, Shamim K, Dubey SK. Biological characterization of lead resistant bacteria to explore role of bacterial metallothioneinin lead resistance, Current Science. 2012 Aug; 103(4):1–3.

  • Naik MM, Khanolkar DS, Dubey SK. Lead resistant Providentia alcalifa-ciens strain 2EA bioprecipitates Pbþ2 as lead phosphate, Letters in Applied Microbiology. 2013 Nov; 56(2):99–104. https://doi.org/10.1111/lam.12026. PMid: 23163530.

  • Marrero-Coto J, Díaz-Valdivia A, Coto-Pérez O. Mecanismos moleculares de resistencia a metales pesados en las bacterias y sus aplicaciones en la biorremediación, Revista CENIC. Ciencias Biológicas. 2010; 41(1):67-78.

  • Oliveira M, Santos T, Vale H, Delvaux J, Cordero P, Ferreira A, Miguel P, Totola M, Costa M, Moraes C, Borges A. Endophytic microbial diversity in coffee cherries of Coffea arabica from south eastern Brazil; Canadian Journal of Microbiology. 2013 Jan; 59(4):221-30. https://doi.org/10.1139/cjm-2012-0674. PMid: 23586745.

  • Blackwood CB, Oaks A, Buyer JS. Phylum- and class-specific PCR primers for general microbial community analysis, Applied and Environmental Microbiology. 2005; 71(10):6193–98. https://doi.org/10.1128/AEM.71.10.6193-6198.2005. PMid: 16204538, PMCid: PMC1265930.

  • Rathnayake IVN, Mallavarapu M, Krishnamurti GSR, Bolan NS, Naidu R. Heavy metal toxicity to bacteria -Are the existing growth media accurate enough to determine heavy metal toxicity, Chemosphere. 2013 Jan; 90(3):1195−200. https://doi.org/10.1016/j.chemosphere.2012.09.036. PMid: 23040649.

  • Zhang YF, He LY, Chen ZJ, Zhang WH, Wang QY, Qian M, Sheng XF. Characterization of lead-resistant and ACC deaminase-producing endophytic bacteria and their potential in promoting lead accumulation of rape, Journal of Hazardous Materials. 2011 Feb; 186(2-3):1720−25. https://doi.org/10.1016/j.jhazmat.2010.12.069. PMid: 21227577.

  • Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores, Analytical Biochemistry. 1987 Feb; 160(1):47−56. https://doi.org/10.1016/0003-2697(87)90612-9.

  • Kamika I, Momba, MN. Assessing the resistance and bioremediation ability of selected bacterial and protozoan species to heavy metals in metal-rich industrial waste-water, BMC Microbiology. 2013 Feb; 13−28. https://doi.org/10.1186/1471-2180-13-28.

  • Pérez-Cordero A, Barraza-Román Z, Martínez-Pacheco D. Identificación de bacterias endófitas resistentes a plomo, aisladas de plantas de arroz. Agronomía, Mesoamericana. 2015; 26(2):267−76.

  • Pérez A, Martínez D, Barraza Z, Marrugo J. Bacterias endófitas asociadas a los géneros Cyperus y Paspalum en suelos contaminados con mercurio, Revista U.D.C.A Actualidad and Divulgación Científica. 2016; 19(1):67−76.

  • Ramirez-Ramirez A, Benítez - Campo N. Tolerancia y reducción de cromo (VI) por Bacillus cereus B1, aislado de aguas residuales de una curtiembre, Revista de Ciencias. 2013; 17(2):51−63.

  • Ma Y, Oliveira R, Nai F, Rajkumar M, Luo Y, Rocha I, Freitas H. The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil, Journal of Environmental Management. 2015 Jun; 156:62−69. https://doi.org/10.1016/j.jenvman.2015.03.024. PMid: 25796039.

  • Ma Y, Prasad M, Rajkumar M, Freitas H. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils; Biotechnology Advances. 2011 Mar; 29(2):248−58. https://doi.org/10.1016/j.bio-techadv. 2010.12.001. PMid: 21147211.

  • Rajkumar M, Noriharu A. Freitas H. Endophytic bacteria and their potential to enhance heavy metal phytoextraction, Chemosphere. 2009 Sep; 77(2):153−60. https://doi.org/10.1016/j.chemosphere.2009.06.047. PMid: 19647283 .

  • Naik MM, Dubey SK. Lead-enhanced siderophore production and alteration in cell morphology in a Pb-resistant Pseudomonas aeruginosa strain 4EA, Current Microbiology. 2011 Feb; 62(11):409–14. https://doi.org/10.1007/s00284-010-9722-2. PMid: 20661573.


Abstract Views: 196

PDF Views: 0




  • Lead Resistance by Bacillus cereus 1DH1LIM Isolated from Contaminated Environments with Mercury

Abstract Views: 196  |  PDF Views: 0

Authors

Alexander Pérez-Cordero
Sucre University, Agricultural Sciences, Agricultural Bioprospecting Group. Campus Universitario Puerta Roja, AA 406, Sincelejo - Sucre, Colombia
Andrea Pérez- Espinosa
Sucre University, Health Sciences, Campus Universitario Puerta Blanca, AA 406, Sincelejo - Sucre, Colombia
Deimer Vitola -Romero
University of Sucre, Education and Sciences, Bioprospecting Agricultural Group. Campus Universitario Puerta Roja, AA 406, Sincelejo, Sucre, Colombia

Abstract


Objective: Evaluated resistance capacity in vitro of Bacillus cereus 1DH1LIM at different concentrations of lead in the form of Pb (NO3)2. Materials and Statistical Analysis: B. cereus was purified, aliquots of suspensions in log phase were inoculated into minimal medium tris-MMT with different concentrations of lead in the form of Pb (NO3)2 and incubated by stirring at 150 rpm at 32 °C for 120 hours; growth was determined by turbidimetry at 600 nm every hour for four days. Siderophore production was determined by growth on médium azurol-S (CAS). Findings: B. cereus 1DH1LIMIt shows statistical difference with respect to adaptation time and concentration of lead metal present in the medium. At concentrations of 100, 150 and 200 ppm of Pb an adaptation phase of 4 hours, with respect to concentrations of 250 to 400 ppm which lasted 7 hours was observed. The highest growth of B. cereus 1DH1LIMIt was observed at 100 ppm, 150 ppm and 200 ppm and less at400. In CAS medium the bacterial culture exhibited siderophore production. Applications: The findings of this study expand the knowledge to use this endophytic bacteria asa biological resource to remedy lead-contaminated environments.

References





DOI: https://doi.org/10.17485/ijst%2F2018%2Fv11i38%2F131974