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Phytochemical Screening, Quantification of Total Phenols, Total Flavonoids and Antimicrobial Activity of Stem Extracts of Salacia Oblonga


Affiliations
1 Sathyabama Institute of Science and Technology, Chennai – 600119, Tamil Nadu, India
2 Vel Tech Multi Tech Dr. Rangarajan and Dr. Sakunthala Engineering College, Chennai – 600 062, Tamil Nadu, India
 

Salacia oblonga, an endangered medicinal plant widely used for the traditional ayurvedic medicine, belonging to the family Clastraceae. Our present investigation revealed the preliminary phytochemical analysis, total phenols, total flavonoids and antimicrobial activities of aqueous stem extract of salacia oblonga. The stem extracts were prepared by using the solvents like ethanol, acetone, petroleum ether, chloroform and water. Preliminary screening of the extracts was carried out by standard procedures. Among the five different solvents, the aqueous stem extract shown more positive for the availability of natural chemical components. Total phenols and total flavonoids were quantified as 35.63 mg GAE/g and 19.82mg QE/g respectively. The aqueous stem extract was tested against the micro-organisms (Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, Staphylococousaureus and Escherichia coli). Among the micro-organisms Bacillus subtilis shown maximum zone of inhibition (13.2mm) at a concentration of 30mg/ml of aqueous stem extract of salaciaoblonga. But Escherichia coli inactive against the stem extract.
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  • Phytochemical Screening, Quantification of Total Phenols, Total Flavonoids and Antimicrobial Activity of Stem Extracts of Salacia Oblonga

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Authors

C. Gladis Raja Malar
Sathyabama Institute of Science and Technology, Chennai – 600119, Tamil Nadu, India
C. Chellaram
Vel Tech Multi Tech Dr. Rangarajan and Dr. Sakunthala Engineering College, Chennai – 600 062, Tamil Nadu, India

Abstract


Salacia oblonga, an endangered medicinal plant widely used for the traditional ayurvedic medicine, belonging to the family Clastraceae. Our present investigation revealed the preliminary phytochemical analysis, total phenols, total flavonoids and antimicrobial activities of aqueous stem extract of salacia oblonga. The stem extracts were prepared by using the solvents like ethanol, acetone, petroleum ether, chloroform and water. Preliminary screening of the extracts was carried out by standard procedures. Among the five different solvents, the aqueous stem extract shown more positive for the availability of natural chemical components. Total phenols and total flavonoids were quantified as 35.63 mg GAE/g and 19.82mg QE/g respectively. The aqueous stem extract was tested against the micro-organisms (Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, Staphylococousaureus and Escherichia coli). Among the micro-organisms Bacillus subtilis shown maximum zone of inhibition (13.2mm) at a concentration of 30mg/ml of aqueous stem extract of salaciaoblonga. But Escherichia coli inactive against the stem extract.

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DOI: https://doi.org/10.17485/ijst%2F2018%2Fv11i23%2F125632