Segmentation and Extraction of Chromosomes from G-Band Metaphase Images

Objectives: Genetic problems can be identified by a test called Karyotyping. Karyotyping is used to find the abnormalities and genetic disorders or defects. The automated segmentation and disentangling of chromosomes are the challenging issues. Methods/Statistical Analysis: In current systems the separation between touching and overlapping chromosomes need human intervention. Different procedures are proposed to obtain the chromosome from its background. In this paper, an attempt is made to separate the chromosomes for Karyotyping in G-Band metaphase images. As a first step segmentation of chromosomes is done. Findings: Thesis carried out by means of local thresholding, region growing and edge detection methods. Then, the chromosome and its clusters are extracted by labelling the connected components. This process successfully separates individual chromosomes. Application: Medical image Processing.