Indian Journal of Science and Technology

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Objective: To identify discarded dental tissues as source for stem cells isolation and their differentiation into neurogenic lineage for regenerative applications. Methods/Analysis: Dental pulp tissues (Both impacted and erupted) were collected from 18-40 years old individuals having proper orthodontic reasons. The long-term in vitro culture of dental pulp stem cells (DPSCs) was established in controlled condition. Population doubling analysis was performed at different passages. DPSCs were further triggered into neurogenic cells under serum free retinoic acid defined supplements. Neurospheres development and neurogenic lineage differentiation was identified. Findings: More than 80% DPSCs were found to express putative dental stem cell marker CD90. We observed all basic biological characteristics of DPSCs during long-term cultivation up to 20 passages. The diameter distribution of cultured DPSCs was quite stable and was found to be 12-18μm in diameter. Highest numbers of neuropsheres were found to be at day 14 with ~100μm in size. Expression of β tubulin-III revealed highest differentiation (>15%) towards neuronal cells. Novelty/Improvement: The present study demonstrates that DPSCs could be a potential option to make repository of neurogenic cells for regenerative applications.

Keywords

Neural Crest Cells, Neural Lineage Cells, Neurospheres, DPSCs, PDT
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