Objectives: To study the potential of callus cultures of Vigna radiata for the biosynthesis of silver nanoparticles. Methods: Callus cultures of Vigna radiata were established in MS media and the aqueous extract of callus cultures was used for biogenic synthesis of silver nanoparticles using 1 mM AgNO3 at ambient temperature.The nanoparticles were characterized by UV-visible spectroscopy and scanning electron microscopy. Findings: This is the first report of use of callus cultures of Vigna radiata for the biogenesis of silver nanoparticles.The synthesis was rapid and completed in 2 hours. UV-visible spectrophotometry revealed an absorbance peak at 455 nm. Scanning electron microscopy revealed the presence of a high density of spherical or ellipsoidal silver nanoparticles. Phytochemical studies revealed the presence of phenolics in the extract which could have played a role in the formation of silver nanoparticles. The findings are important since this is a novel approach and there are very few reports of synthesis of silver nanoparticles using callus cultures. Application: Callus cultures are more suitable for biosynthesis of bio-compatible nanoparticles as compared to microbes or plant parts since these are generated axenically and are renewable resources that are easy to authenticate.
Keywords
Callus, Green Gram, Silver Nanoparticles, Vigna radiata
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