Fuel methanol is on demand nowadays. Lignocelluloses and algal biomasses are largely generated as waste materials out of agricultural practises and process industries. In the present work, methanol production by Saccharomyces cerevisiae was carried out using lignocellulosic biomass (vegetable) as a substrate and using rotating biological reactor that utilizes biologically pretreated vegetable as substrates for methanol production and its process optimization. During the batch experiment with varying substrate concentration (60-100 g/l), pH (4.8-5.8) and commercial cellulase enzyme concentration of (10- 20 mg/ml) resulted in 38.9 g/l of methanol concentration with a maximum of 17 % yield. The SEM analysis was carried out for analysing the structural morphology of the untreated, pretreated, hydrolysed and fermented samples.
Keywords
Agricultural Waste, Biological Reactor, Lignocellulosic Biomass, Methanol, Optimization, Vegetable
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