Raghwendra Singh , Meenal Rathore , Gyan P. Mishra , Meetul Kumar , Rajio Singh , Z. Ahmed

Abstract

In the present investigation four different explants from Tecomella undulata were used for in vitro adventitious shoot regeneration. The treatment having IAA (0.1 mg/l) and zeatin (2.5mg/l) in combination yielded maximum number of shoots from hypocotyle (16) and cotyledonary nodes (27). In case of epicotyle, the best response (11 shoots per explant) was observed in the treatments having IAA (0.1 mg/l) + BAP (2.5 mg/l). While, regeneration of shoots from cotyledon was not observed. Pretreatment of regenerated shoots in a mixture of NAA, IAA and IBA (5.0 mg/l, each) for 36 hours was found to be the best for ischolar_main induction. Survival of 75% was observed from the plantlets kept for hardening, whereas 62% hardened plants survived in field. For transformation, continuous light and mild shaking with hands at three to five hours interval for 36 hours was found more efficient (5% transformation). Hence, we report here an efficient adventitious shoot regeneration system in T. undulata using cotyledonary node as an explant that has a vital role in gene transfer technologies.

Keywords

Rohida (Tecomella undulata), Adventitious Shoot Regeneration, Agrobacterium
tumefaciens, Transformation

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Ranjit Singh , P. S. Negi , M. C. Arya , Z. Ahmed

Abstract

The study standardized the propagation techniques of Crataegus crenulata through seeds and cuttings. Mature pencil thick cuttings were collected and treated with different hormone concentrations (100, 150, 200, 250, 300 nd 350 ppm) of hormones i.e. IBA (Indole -3-butyric acid), IAA (Indole-3-acetic acid) and NAA (Napthelene-6- acetic acid). Sprouting and ischolar_maining capacity of the cuttings were assessed under open environmental conditions. Data revealed that IBA 250 ppm significantly enhanced sprouting, ischolar_maining and ischolar_main length as compared to other plant growth regulators and control. The IBA (250 ppm) treated cuttings were found to be best for maximum plant length (189.32cm) and number of branches (6.45) after six months of transplantation in the field. Overnight (12 hr) dipping the cuttings in running water was observed helpful in removing phenolic components. Dipping of seeds in lukewarm water overnight (12 hrs) significantly improved in germination per centage (85-90 %) as compared to control (65-70%). Comparative study indicate that cutting raised seedlings were having edge over seed raised seedlings in terms of plant height and number of branches and being a faster method of multiplication also.

Keywords

Crataegus crenulata, IAA, IBA, NAA, Ghingaroo

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Anuja Kumari , M. C. Arya , P. K. Joshi , Z. Ahmed

Abstract

Jatropha curcas, commercially important tree species used to produce biofuels, is known for its multipurpose benefits and its role in agro forestry. Present study examines the amenability of different J. curcas strains viz. DARL-1, DARL-2, CJC-18 and CJC-19 through semi hardwood cuttings treated with hormones [Indole 3 acetic acid, α- Naphthalene acetic acid and Indole 3 butyric Acid (200ppm)] .The exogenous plant hormones significantly affected the cuttings of different cultivars in early emergence of shoots, shoots number, shoot length (cm) and leaves per cutting as recorded in the study. The effectiveness was in the order IBA>NAA>IAA when applied to the cuttings. Among the different strains DARL-2 followed by DARL-1 showed better response to hormones as compared to CJC-18 and CJC-19. Over all, it was concluded that semi hardwood cuttings of DARL-2 and DARL-1 treated with IBA 200ppm can be used for the mass propagation of J. curcas under Central Western Himalayas of Uttarakhand.

Keywords

Jatropha curcas, Hormones, Semi Hardwood Cuttings, IBA

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