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Dobhal, Shivani
- Genetic Diversity in Dalbergia sissoo Roxb. Through Molecular Markers for Practical Applications
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Indian Forester, Vol 138, No 9 (2012), Pagination: 819-821Abstract
The tree improvement programme of Dalbergia sissoo Roxb. was started by selecting about 300 plus trees from the distribution range by adopting index method of selection. The selected plus trees were cloned through ischolar_main suckers and single nodal cuttings ischolar_mained both in mist and mist-less chamber. Genetic diversity of further selected forty nine clones was tested through DNA based molecular marker. A total of thirty decamer random amplified polymorphic DNA (RAPD) primers initially screened which were later reduced to ten most informative primers for the evaluation of level of genetic diversity. The primers generated 129 reproducible RAPD bands with fragments ranging from 200 to 1300 base pairs, of which 110 were polymorphic. The polymorphic information content (PIC) among the clones varied from 0.154 (M-33) to 0.258 (M-122) to witness that RAPD primers detected genetic diversity among the clones. The highest polymorphism (93.33 %) was reported with primer OPAG-16 and the lowest (63.63 %) with primer OPAF-16. Nonetheless, the average polymorphism with ten primers was calculated to 84.93 %. The studies would play a complementary role in hybridization, when selection of most divergent parents becomes supreme importance to harness maximum heterosis in the shortest possible time.Keywords
Dalbergia Sissoo, Rapd, Genetic Diversity, Polymorphism And Heterosis- Shisham Mortality in Hoshiarpur, Punjab: Causes and Remedy
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Authors
M. S. Bhandari
1,
Rama Kant
1,
Nafeesh Ahmed
1,
Shivani Dobhal
1,
R. K. Luna
2,
S. Nautiyal
1,
Vijay Kumar
,
Ashok Kumar
1
Affiliations
1 Division of Botany, FRI, Dehradun-248 006, Uttarakhand, IN
2 Punjab Forest Department, Hoshiarpur, Punjab, IN
1 Division of Botany, FRI, Dehradun-248 006, Uttarakhand, IN
2 Punjab Forest Department, Hoshiarpur, Punjab, IN
Source
Indian Forester, Vol 140, No 2 (2014), Pagination: 147-153Abstract
Dalbergia sissoo (Shisham) is an important tree species for production of quality timber. The genetic diversity of shisham is very low; which has perhaps resulted in increased infestation by diseases and insect-pest attack. The species is eclipsed with one of the most important disease called dieback wilt caused by Fusarium solani f. sp. dalbergiae. The biotic and abiotic factors help in the spreading of diseases in the plantation trials of shisham. In the plantation trial at Hoshiyarpur (Punjab) the dieback wilt causes the tremendous damage to standing trees of shisham. Mortality was found highest (7.65%) for the experimental trials of shisham that includes a total of 49 clones and rametes. The disease was spreading rapidly and affecting the other healthy plantation of the trials. The serious preventive management efforts and mitigation measures are required to protect the shisham trials.Keywords
Agro-forestry, Diseases, Insect-pest and Genetic Diversity.References
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- Evaluation of Clonal Divergence in Dalbergia Sissoo Roxb. for Developing Production Populations
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Authors
Affiliations
1 Division of Genetics and Tree Propagation, Forest Research Institute, Dehradun, IN
2 National Academy of Agricultural Research Management (NAARM), Hyderabad, Andhra Pradesh, IN
1 Division of Genetics and Tree Propagation, Forest Research Institute, Dehradun, IN
2 National Academy of Agricultural Research Management (NAARM), Hyderabad, Andhra Pradesh, IN
Source
Indian Forester, Vol 142, No 5 (2016), Pagination: 451-458Abstract
Forty eight clones of Dalbergia sissoo Roxb. were subjected to genetic divergence studies using Mahalanobis D2 statistics. The clones were field planted in two geographical locations at Hissar, Haryana (N 29 30°17.7" E 75°33' 14'') and Hoshiarpur, Punjab (N31° 33' 31.7" E 75° 49' 0.5") to analyze different genetic parameters and divergence. At the age of eighteen months, height ranged from 41.17 cm (Clone 94) to 112.52 cm (Clone 5003) and collar diameter ranged from 0.59 cm (Clone 31) to 2.02 cm (Clone 5006). Mean value based on both primary quantitative traits expressed superiority of thirteen clones over the locations. Using Tocher's method 48 genotypes were grouped into nine clusters, whereas Cluster III and VI consists maximum eight clones each. Cluster VIII was reported to be a solo cluster. The maximum D2 value (1.62) was observed between Clone 1003 (Uttarakhand) and Clone 5007 (Uttarakhand). It was therefore obvious that these divergent clones could be planted in seed orchards so that high degree of heterosis could be exhibited. Additionally such clones could possibly play an important role in production of new recombinants with maximum acquisition of traits of economic importance.Keywords
Genetic Parameters, Divergence, Clustering, Quantitative Traits, Heterosis.References
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