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Joshi, Kirti
- Population Assessment of Trichoderma piluliferum Using a Modified Selective Medium
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Indian Forester, Vol 135, No 7 (2009), Pagination: 960-964Abstract
Trichoderma species are one of the potential biocontrol agents exploited to manage many of the soil and seed borne plant pathogens. Quantitative estimation of Trichoderma species in soil is often difficult because of relatively rapid growth of other soil fungi on conventional agar media, so selective medium is required for isolating Trichoderma from soil as well as for estimating its survival in soil. Various media and different combinations have been tried for making dilution plates of Trichoderma from soil. This study was taken up to prepare the most effective selective medium which contained Magnesium sulphate (0.2 g), Potassium orthophosphate (0.9 g), Ammonium nitrate (1.0 g), Potassium chloride (0.15 g), Glucose (3.0 g), PCNB (0.1 g), Rose Bengal (0.1 g), Chloramphenicol (0.25 g), Agar (15 g), Metalaxyl (0.1 g) and Distilled water (1 lit).Keywords
Trichoderma-specific Medium, Trichoderma piluliferum
- Mycoflora Associated with Different Seed Sources of Pinus wallichiana
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Indian Forester, Vol 130, No 12 (2004), Pagination: 1410-1415Abstract
Seed samples from different sources of Pinus wallichiana from Himachal Pradesh and Uttaranchal were evaluated for the occurrence of seed mycoflora. A total of 13 fungi with 22 fungal species were isolated of which species of Aspergillus and Penicillium were dominant. Seed source Kufri (H.P.) reported maximum number of mycoflora i.e. 9 fungi, while in Sawat and Kharapathar (H.P.) only one fungus was recorded namely Aspergillus niger.- Evaluation of Seed-Borne Fungi of Pinus roxburghii
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