Arvind Shukla , Hariom Kumar Sharma

Abstract

no abstract

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Gargi Joshi , Arvind Shukla , Alok Shukla

Abstract

A study was conducted to assess,the Influence of different plant growth hormones on physiology of flowering in Jatropha curcas L. variety Pant- J Selection 2 as flowers have the major importance on seed yield and thus biodiesel production. Application of plant growth regulators such as Ethrel, IAA and NAA as foliar spray was found to influence the flowering pattern of plant. Higher concentrations (100 ppm and 150 ppm) of all plant growth regulators had a more prominent effect on flowering and significantly increased the number offemale flowers of the treated plants.

Keywords

Flowering, Jatropha Curcas, Plant Growth Hormones

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Anjani , Arvind Shukla , Alok Shukla , S. K. Guru

Abstract

Two types of callus proliferation namely, embryogenic (E) and non-embryogenic (NE) calli were obtained by culturing 2 to 3 days old leaf of Jatropha curcas L. (Pant Jatropha Selection-II) in modified MS medium supplemented with 2 ppm 2,4-D, and 0.5 ppm BAP. Scanning electron micrographs indicated distinct morphology of cell shape. NE cell shape was tubular and loosely arranged cells. Embryogenic callus comprised nodular or knobby, quite deep embedded and tightly packed cell mass. Histological observations of callus sections revealed that E callus occurred on the surface as well as in the deeper regions of the callus. The abundance of vessel tracts in the callus was consistent with high content of vessel elements, rounded and cytoplasmically dense cells with totipotency. This may imply that nutrients are easily transported via these vessel tracts for the nourishment of growing somatic embryos or leafy structure. The number of vessel elements appeared to be a crucial factor in determining the E and NE calli development.

Keywords

Embryogenic callus, Totipotency, Vessel tracts, Somatic embryos, In vitro organogenesis

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