In Vitro Propagation of Haldu (Adina cordifolia Roxb.)

Haldu (Adina cordifolia Roxb.) is a naturally grown tree in moist deciduous forests of Bangladesh and has diversified uses. But the population of the species has become threatened due to over exploitation. The large scale propagation of this species is quite difficult through sexual and asexual methods. So to rescue this important species from going to be threatened, attempts were made to develop an efficient protocol through in vitro culture that would maintain and propagate it in large scale. The shoot tips were used as explants and culture established in MS media supplemented with different concentration of BAP and combination of BAP and NAA 0.5 mg/l. It was observed that MS media supplemented with BAP 1.0 + NAA 0.5 mg/l was more effective for shoot production and development. The present study also showed that IBA was more suitable than NAA where IBA 1.0mg/l was found more effective for ischolar_main induction. After sequential hardening, ischolar_mained plantlets were successfully transferred to soil and the plantlets were grown luxuriously.