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Gupta, Sudha
- Ancient DNA – Pitfalls and Prospects
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Authors
Affiliations
1 Departments of Botany, Pteridology-Palaeobotany Section, University of Kalyani, Kalyani - 741235, West Bengal, IN
2 Departments of Botany, Cytogenetics, Genetics and Plant Breeding Section, University of Kalyani, Kalyani - 741235, West Bengal, IN
1 Departments of Botany, Pteridology-Palaeobotany Section, University of Kalyani, Kalyani - 741235, West Bengal, IN
2 Departments of Botany, Cytogenetics, Genetics and Plant Breeding Section, University of Kalyani, Kalyani - 741235, West Bengal, IN
Source
Indian Journal of Science and Technology, Vol 8, No 13 (2015), Pagination:Abstract
Ancient DNA (a-DNA) studies in past three decades has been uplifted from short stretch archaeological mtDNA retrieval to reconstruction of complete mitochondrial genome sequence using next generation sequencing. Study of a-DNA acts as validating tool to test the assumed hypothesis used in origin, evolution, diversification, distribution, domestication and extinction of species; although, scientific progression in the field is frequently hindered by erroneous reports due to contamination and other technical bottlenecks. With the advent of appropriate methodologies such as 3’-C3 spacer tagged oligonucleotide blocking, shotgun sequencing, amplicon sequencing Polymerase Chain Reaction, single primer extension methodology, development of broad spectrum ancient sequence primer, slippage proof amplification methods, use of multiplexing strategy in PCR (Polymerase Chain Reaction) amplification, etc. in a-DNA research try to minimize the difficulties associated with it. Present review highlighted the pitfalls as well as prospects of a-DNA study and tries to assess the significance and acceptability of a-DNA research for its future application in understanding of evolutionary biology.Keywords
a-DNA, Extraction Difficulties, Modern Technologies, Retrospect of a-DNA- Paleocene-Eocene Tropical Coastal Palynoassemblage from the Older Cenozoic Sediments of Ganga Basin with Records of Recycled Microfossils
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Authors
Affiliations
1 Department of Botany, University of Calcutta, Kolkata - 700 019, IN
2 Department of Botany, University of Calcuttta, Kolkata - 700 019, IN
1 Department of Botany, University of Calcutta, Kolkata - 700 019, IN
2 Department of Botany, University of Calcuttta, Kolkata - 700 019, IN
Source
Journal of Geological Society of India (Online archive from Vol 1 to Vol 78), Vol 65, No 5 (2005), Pagination: 587-596Abstract
Palynological investigation has been undertaken with tile subsurface Cenozoic sediments of Ganga basin to establish biostraligraphy and environment of deposition. Samples from four bore holes viz. Puranpur, Matera, Gandak and Raxaul under study have yieldcd palynomorphs and other microfossils. Palynoassemblage recovered from the older Cenozoic sedimcnts of thc four bore holes has becn analyzed in this paper to ascertain the age and environment of deposition. Rich palynoasscmblage recovered i'rorn the Ganga basin sediments compare closely with the palynoassemblage recovered from Palcoccne-Eocene sediments of other Tertiary basins in India. Occurrence of triporate 'Normapolles' type pollen grains in the assemblage is additional significant record. The palynoassemblage suggests a predominantly tropical warm humid climate with coastal brackish water influence. In addition, reworked microfossils of early and late Paleozoic, Mesozoic ages have becn recovered in the lower Tertiary palynoassemblage.Keywords
Palynoassemblage, Palaeocene-Eocene, Reworked Palynomorphs, Ganga basin.- Need Based Students' Scholarship: PUSH KJSCE - A Success Story
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Authors
Sudha Gupta
1,
Ravindra Salvi
2,
Deepak Sharma
3,
Sonali Patil
4,
H. N. Bharathi
3,
Swati Mahajan
5,
Manoj Palsodkar
6
Affiliations
1 Department of Electronics Engineering, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
2 Department of Science and Humanities, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
3 Dept. of Computer Engg., KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
4 Dept. of Information Technology, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
5 Dept. of Electronics and Telecommunication Engg., KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
6 Dept. of Mechanical Engineering, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
1 Department of Electronics Engineering, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
2 Department of Science and Humanities, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
3 Dept. of Computer Engg., KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
4 Dept. of Information Technology, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
5 Dept. of Electronics and Telecommunication Engg., KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN
6 Dept. of Mechanical Engineering, KJ Somaiya College of Engineering, Vidyavihar, Mumbai, IN