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D. Saraswathi ¹, G. P. Gigi ¹, Ajithadas Aruna ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Madras Medical College, Chennai-600003, IN

Abstract

Two simple, sensitive and accurate spectrophotometric methods have been developed for the estimation of Acebrophylline in bulk and in pharmaceutical formulations (Method A&Method B). Method A is based on the formation of orange red colored chromgen with ferric chloride in the presence of 2,22-bipyridyl which obeys beer's law in the concentration range of 10-100 μg/ml exhibiting maximum absorption at 522nm. Method B is based on the diazotization of Acebrophylline with nitrous acid. The diazonium salt formed was then coupled with â-naphthol which shows maximum absorption at 456nm (yellow colored chromogen). It obeys Beer's law in the concentration ranging from 50-300 μg/ml. The methods were extended to capsule formulation and there was no interference from excipients and diluents. These methods have been statistically validated and are found to be precise and accurate.

Keywords

Acebrophylline (ACE), Ferric chloride, 2,22-Bipyridyl, Diazotization, α-Naphthol.

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V. Niraimathi ¹, V. Prema ¹, Ajithadas Aruna ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai - 600 003, Tamil Nadu, IN

Abstract

Four new, simple, sensitive and reproducible spectrophotometric methods have been developed for the estimation of cefditoren pivoxil in tablet dosage form. Method A involves the determination of cefditoren pivoxil by Standard absorbance method at 230nm and the Beer's concentration range was found to be 5-50 μg/mL. Method B and Method C involve the determination of cefditoren pivoxil by first derivative spectrophotometry and second derivative spectrophotometry respectively. The normal spectrum was derivatized to first and second order derivative spectrum and the linearity was found to lie within the Beer's range for cefditoren pivoxil. Method D involves the determination of cefditoren pivoxil by area under curve method and the linearity was established.

Keywords

Cefditoren Pivoxil(CP), Beer's Law, Standard Absorbance Method, Derivative Spectrophotometry, Area under Curve(AUC).

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M. S. Uma Shankar ¹, Ajithadas Aruna ², N. V. Satheesh Madhav ³, M. K. Ranganath ⁴

Affiliations
1 Department of Pharmaceutical Technology, Krupanidhi College of Pharmacy, #12/1, Chikkabellandur, Carmelaram Post, Bengaluru - 560 035, Karnataka, IN
2 Department of Pharmaceutical Chemistry, Madras Medical College, Park Town, Chennai-600 003, IN
3 Department of Pharmaceutics, Dehradun Institute of Technology, Faculty of Pharmacy, Mussoorie-Diverson Road, Makkawala, P.O. Bhawantpur, Dehredun (Uttarakhand), IN
4 Department of Pharmaceutical Analysis, Krupanidhi College of Pharmacy, #12/1, Chikkabellandur, Carmelaram Post, Bengaluru - 560 035, Karnataka, IN

Abstract

Two simple, validated and sensitive spectrophotometric methods have been developed for the estimation of nicorandil in bulk and in pharmaceutical formulation (Method A&Method B). The method A involves the determination of nicorandil by standard absorbance method at 262 nm which obeys Beer's law in concentration range of 20-120 ì g/mL. Method B is based on the formation of intensely yellow ammonium salts by the interaction of nicorandil with phenol-2,4-disulphonic acid which obeys Beer's law in concentration range of 10-60 μg/ml exhibiting maximum absorption at 405 nm under the optimized experimental conditions. The results obtained by the proposed method are acceptable. The methods were extended to tablet formulation and there was no interference from excipients and diluents. These methods have been statistically validated and are found to be precise and accurate.

Keywords

Nicorandil (NIC), Phenol-2,4-Disulphonic Acid, Yellow Ammonium Salt, Beer's Law.

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