Two simple, sensitive and accurate spectrophotometric methods have been developed for the estimation of Acebrophylline in bulk and in pharmaceutical formulations (Method A&Method B). Method A is based on the formation of orange red colored chromgen with ferric chloride in the presence of 2,22-bipyridyl which obeys beer's law in the concentration range of 10-100 μg/ml exhibiting maximum absorption at 522nm. Method B is based on the diazotization of Acebrophylline with nitrous acid. The diazonium salt formed was then coupled with â-naphthol which shows maximum absorption at 456nm (yellow colored chromogen). It obeys Beer's law in the concentration ranging from 50-300 μg/ml. The methods were extended to capsule formulation and there was no interference from excipients and diluents. These methods have been statistically validated and are found to be precise and accurate.
Keywords
Acebrophylline (ACE), Ferric chloride, 2,22-Bipyridyl, Diazotization, α-Naphthol.
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