Synthesis, Characterization and Analysis of the Effect of Copper Oxide Nanoparticles in Biological Systems

Objectives: In the present study, synthesis of copper oxide nanoparticles was prepared by chemical reduction method. This synthesized metal oxide nanoparticles were targeted to study the antibacterial activity against Escherichia coli and Staphylococcus aureus. Methods: The antibacterial activity against Escherichia coli and Staphylococcus aureus was seen by resazurin dye reduction method and disc diffusion method. Further, the effects of nanoparticles on enzyme activity (β- glucosidase) were seen in Escherichia coli. The physical interaction between metal oxides nanoparticles with protein (Ovalbumin) were studied to interrupt the conjugate forming properties. Toxicity studies of the metal oxide nanoparticles on human pathogens open the door for a new range of antibacterial agents. Results: There is a lot of interest among the researchers for developing various protocols for the fabrication of other metal nanoparticles. However, these have been a constant research regarding the use of biological systems, friendly and rapid techniques. Conclusion: The potential application of the nanomaterials-protein conjugates are limited only to novel techniques such as therapeutics in cancer, as drug delivery vehicles, smart nanocomposites, in bioremediation of industrial effluents, biodegradation of effluents and for water purification.