Background:Inflammation is a complex process of various mediators. When huge amount of nitric oxide is produced by inducible nitric oxide synthase (iNOS) which leads to the vasolidation and hypotension that usually observed during septic shock and inflammation. So the inhibitors of iNOS is useful for the treatment of inflammatory diseases.
Methods: Walsura trifoliata, known for antimicrobial and antioxidant activities was tested for anti-inflammatory activity to isolate its bioactive compound. The major fractions obtained from the methanol extract of the ischolar_main of Walsura trifoliate using column chromatography was screened for their inhibitory activity against NO production in lipopolysaccharide (LPS) activated macrophages.
Findings:A total of 79 fractions were isolated and based on TLC, similar fractions were combined together to form 8 major fractions. Among the eight major frcations, fractions 4 and 7 showed potent anti-inflammatory activity. Significant cytotoxicity was also observed in fractions 4 and 7. Using Infrared spectroscopy and Mass spectroscopy analysis fraction 4 was identified as 3,4,-5 trihydroxy benzoic acid and fraction 7 was identified as a-sitosterol.
Application:Further in vivo studies should be undergone for the development of drug from the active fractions of Walsura trifoliata for its phytotherapeutical uses in different ailments such as antimicrobial, antioxidant and anti inflammatory diseases.