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In Vitro Rooting in the Tissue Culture Raised Plantlets of Malbhog Cultivar of Banana


Affiliations
1 CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India
2 Ranchi women’s College, Ranchi University, Ranchi - 834001, India
3 Biotechnology Laboratory, University Department of Botany, B.R. Ambedkar Bihar University, Muzaffarpur – 842 001, India
 

Banana plantlets propagated through shoot tip culture were used for in vitro ischolar_maining in ½ strength MS medium supplemented with different concentrations (0.2mg/l - 2.0mg/l) of three different auxins either alone or in combinations. ½ strength MS + 1.0 mg/l IBA + 0.5 mg/l IAA induced ischolar_mains among 90% of the explants after 12th days of inoculation. The average number of ischolar_mains in this condition was 6.0. Increase in the concentrations of the above two auxins had no promoting impact; rather the number of ischolar_mains were reduced. Similarly IBA (1.5mg/l) alone induced ischolar_maining but the percentage response was reduced (66%) and time of initiation was increased (18th days). Lower concentration of IBA (0.5mg/l) + 0.5mg/l IAA had also no promoting impact. About 70% of the explants responded in this after 15th days of incubation. Higher concentration of IBA (1.5mg/l) + 0.5mg/l NAA, revealed reduced rate of response, similarly IBA at its different concentration revealed no better response with NAA at 0.2 or 0.5mg/l.

Keywords

Micropropagation, Plantlets, in Vitro, Explants, Auxins, Innoculation
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  • In Vitro Rooting in the Tissue Culture Raised Plantlets of Malbhog Cultivar of Banana

Abstract Views: 624  |  PDF Views: 62

Authors

Arun Kumar
CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India
Pingal Kumari
Ranchi women’s College, Ranchi University, Ranchi - 834001, India
L. N. Shukla
Biotechnology Laboratory, University Department of Botany, B.R. Ambedkar Bihar University, Muzaffarpur – 842 001, India

Abstract


Banana plantlets propagated through shoot tip culture were used for in vitro ischolar_maining in ½ strength MS medium supplemented with different concentrations (0.2mg/l - 2.0mg/l) of three different auxins either alone or in combinations. ½ strength MS + 1.0 mg/l IBA + 0.5 mg/l IAA induced ischolar_mains among 90% of the explants after 12th days of inoculation. The average number of ischolar_mains in this condition was 6.0. Increase in the concentrations of the above two auxins had no promoting impact; rather the number of ischolar_mains were reduced. Similarly IBA (1.5mg/l) alone induced ischolar_maining but the percentage response was reduced (66%) and time of initiation was increased (18th days). Lower concentration of IBA (0.5mg/l) + 0.5mg/l IAA had also no promoting impact. About 70% of the explants responded in this after 15th days of incubation. Higher concentration of IBA (1.5mg/l) + 0.5mg/l NAA, revealed reduced rate of response, similarly IBA at its different concentration revealed no better response with NAA at 0.2 or 0.5mg/l.

Keywords


Micropropagation, Plantlets, in Vitro, Explants, Auxins, Innoculation

References