Indian Journal of Innovations and Developments

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


The two different laboratory experiments illustrate the evaluation of raffinose family oligosaccharides for the graduate and post graduate students. The thin layer chromatography (TLC) method is the simple and inexpensive method, while high performance liquid chromatography (HPLC) is the most precise and sensitive technique, useful for the both graduate and post graduate students. Raffinose family oligosaccharides (RFOs) are important constituents of a wide variety of grain legumes and are thought to be the major producers of flatulence. Recent researches showed their beneficial effects also. Mung bean (Phaseolus aureus L.) is the principal crop from which edible bean sprouts, noodles and weaning foods are prepared. Two mung bean cultivars analyzed for oligosaccharide content by two different methods; TLC and HPLC, results were compared and discussed. These experiments are mainly intended for the students of plant biochemistry and nutrition.

Keywords

TLC, HPLC, Mung bean, RFOs
User
Notifications