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Optimization and Production of Proteinacious Chicken Feather Fertilizer by Proteolytic Activity of Bacillus Sp. MPTK 6


Affiliations
1 Department of Plant Biology and Plant Biotechnology, Loganatha Narayanasamy Government College, Ponneri, TN, India
2 Department of Biotechnology, KalasalingamUniversity, Virudhunagar, TN, India
3 CAS in Botany, University of Madras, Guindy Campus, Chennai, TN, India
 

This study illustrated that optimization of inorganic nutrients, metal ions and various cost-effective substrates yield a higher amount of solubilised protein which can be recovered from chicken feather powder by proteolytic activity of Bacillus sp. MPTK6. The test organism used in this study was isolated from feather composting soil in keratin enrichment medium at Ph 8.5. The isolate Bacillus sp. MPTK6 showed a clear zone on the keratin enriched medium. Magnesium sulphate at 0.5% yielded high protease production and solubilisation where as KH2PO4 and K2HPO4 exhibited the same effect at 0.3%. Zinc sulphate and ferrous sulphate (15 μg/ml) significantly stimulated the enzyme production and protein yield. Corn steep liquor (0.3%) and peptone (0.3%) exert a very strong stimulatory effect on protease yield as well as protein solubilisation.

Keywords

Solubilised Protein,Bacillus Sp., Magnesium Sulphate, Zinc Sulphate, Ferrous Sulphate
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  • Optimization and Production of Proteinacious Chicken Feather Fertilizer by Proteolytic Activity of Bacillus Sp. MPTK 6

Abstract Views: 417  |  PDF Views: 70

Authors

V. Veerabadran
Department of Plant Biology and Plant Biotechnology, Loganatha Narayanasamy Government College, Ponneri, TN, India
S. Nithya Balasundari
Department of Biotechnology, KalasalingamUniversity, Virudhunagar, TN, India
D. Monica Devi
Department of Biotechnology, KalasalingamUniversity, Virudhunagar, TN, India
D. J. Mukesh Kumar
CAS in Botany, University of Madras, Guindy Campus, Chennai, TN, India

Abstract


This study illustrated that optimization of inorganic nutrients, metal ions and various cost-effective substrates yield a higher amount of solubilised protein which can be recovered from chicken feather powder by proteolytic activity of Bacillus sp. MPTK6. The test organism used in this study was isolated from feather composting soil in keratin enrichment medium at Ph 8.5. The isolate Bacillus sp. MPTK6 showed a clear zone on the keratin enriched medium. Magnesium sulphate at 0.5% yielded high protease production and solubilisation where as KH2PO4 and K2HPO4 exhibited the same effect at 0.3%. Zinc sulphate and ferrous sulphate (15 μg/ml) significantly stimulated the enzyme production and protein yield. Corn steep liquor (0.3%) and peptone (0.3%) exert a very strong stimulatory effect on protease yield as well as protein solubilisation.

Keywords


Solubilised Protein,Bacillus Sp., Magnesium Sulphate, Zinc Sulphate, Ferrous Sulphate

References