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A Preclinical Evaluation on Antioxidant and Gastroprotective Effect of Dioscorea Bulbifera in Wistar Rats


Affiliations
1 Shield health care Pvt Ltd, Chennai-600095, Tamil Nadu, India
2 SASTRA University, Tanjavur-613401, Tamil Nadu, India
 

The present study was undertaken to evaluate the gastro-protective effect of hydro alcohol extract of Dioscorea bulbifera (DB) tubers Linn in indomethacin-induced gastric ulcers in rats at doses of 100, 200 and 400 mg/kg body weight. The gastric lesions were significantly reduced by all doses of DB as compared with the Indomethacin (100 mg/kg body weight) treated group. In the stomach tissues of treated animals, the in-vitro antioxidant levels were evaluated. The administration of indomethacin caused a significant decrease in the levels of peroxidase, catalase (CAT) and an increase in glutathione peroxidase (GPx) and reduced glutathione (GSH), and lipid peroxidation (LPO) level. The administration of all doses of DB reversed the trend, inducing a significant increase of peroxidase, Catalase and a reduction in GPx, GSH and LPO level in tissues. These results suggest that the gastro-protective effect of DB can be attributed to its reducing effect on the oxidative damage in rats.

Keywords

Gastro-protective Activity, Dioscorea Bulbifera, Indomethacin, Antioxidant Activity
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  • A Preclinical Evaluation on Antioxidant and Gastroprotective Effect of Dioscorea Bulbifera in Wistar Rats

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Authors

J. Balasubramanian
Shield health care Pvt Ltd, Chennai-600095, Tamil Nadu, India
R. Dhanalakshmi
SASTRA University, Tanjavur-613401, Tamil Nadu, India
P. Jibnomen
SASTRA University, Tanjavur-613401, Tamil Nadu, India
P. Manimekalai
SASTRA University, Tanjavur-613401, Tamil Nadu, India

Abstract


The present study was undertaken to evaluate the gastro-protective effect of hydro alcohol extract of Dioscorea bulbifera (DB) tubers Linn in indomethacin-induced gastric ulcers in rats at doses of 100, 200 and 400 mg/kg body weight. The gastric lesions were significantly reduced by all doses of DB as compared with the Indomethacin (100 mg/kg body weight) treated group. In the stomach tissues of treated animals, the in-vitro antioxidant levels were evaluated. The administration of indomethacin caused a significant decrease in the levels of peroxidase, catalase (CAT) and an increase in glutathione peroxidase (GPx) and reduced glutathione (GSH), and lipid peroxidation (LPO) level. The administration of all doses of DB reversed the trend, inducing a significant increase of peroxidase, Catalase and a reduction in GPx, GSH and LPO level in tissues. These results suggest that the gastro-protective effect of DB can be attributed to its reducing effect on the oxidative damage in rats.

Keywords


Gastro-protective Activity, Dioscorea Bulbifera, Indomethacin, Antioxidant Activity

References