Indian Journal of Innovations and Developments

Open Access Open Access  Restricted Access Subscription Access

Impact of Elevated Temperature on Salmonella typhi


Affiliations
1 Department of Biotechnology, Hindustan College of arts & science, Kelambakkam-603103, Tamil Nadu, India
 

The purpose of this study was to examine the effect of elevated temperature on S.typhi, which is water borne organism and cause the typhoid. It was incubated at various temperatures like 37°C, 42°C, and 47°C for overnight. Then the carbohydrate, lipid and the DNA content in each culture were measured. The results showed that an increase in temperature like 42°C and 47°C produced a marked decrease in the carbohydrates, lipid and DNA content when compared to the culture incubated at 37°C. Therefore slight increase in the temperature caused decrease in carbohydrate, lipid and the DNA content. When the organism is treated at a high temperature that can result in significant decrease in the micro & macromolecules, which ultimately leads to the death of the organism.

Keywords

Samonella typhi, Micro and Macromolecules, Lipids and Carbohydrates
User
Notifications

  • Ahmed N and Rowbury RJ (1971) Temperature-sensitive cell division component in a mutant of Salmonella typhimurium. J Gen Microbiol., 67(1), 107-15.

  • Bryan (1977) Diseases transmitted by food contaminated by wastewater J. Food Prot, 4, 45-56.

  • Crump JA, Youssef FG, Luby SP, Wasfy MO, Rangel JM, Taalat M, Oun SA and Mahoney FJ (2003) Estimating the incidence of typhoid fever and other febrile illnesses in developing countries. Emerg Infect Dis., 9(5), 539-44.

  • Doughari JH (2005) A comparative study on effects of crude extracts of some local medicinal plants and some selected antibiotics on Salmonella typhi. Thesis Federal University of Technology, 5, 1-4.

  • de Jong B, Andersson Y and Ekdahl K (2005) Effect of regulation and education on reptile-associated salmonellosis. Emerg Infect Dis., 11(3), 398-403.

  • Doughari JH, Pukuma MS and De N (2007) Antibacterial effects of Balanites aegyptiaca L. Drel.and Moringa oleifera Lam. on Salmonella typhi. Biotechnol, 6, 2212- 2215.

  • Duguid JP, Marmion BP and Sawin RHA (1983) Mackie and McCartney 13th edn. ELBAS Churhill Livingston. Medical Microbiology, 314-323.

  • John Sommerville (1982) Gene Structure and Expression, Biophysica Acta (BBA), 698,158- 166

  • Pang T, Levine MM, Ivanoff B, Wain J and Finlay BB (1998) Typhoid fever - important issues still remain. Trends in Microbiology, 6, 131–133.

  • Pelczar MJ, Jr Chan ECS and Krieg NR (1993) Microbiology Concepts and Application. 1st ed.

  • Sykes J and Tempest DW (1965) The effect of magnesium and of carbon limitation on the macromolecular organisation and metabolic activity of Pseudomonas sp., Strain. Biochim. Biophy, 9, 103-105.


Abstract Views: 333

PDF Views: 62




  • Impact of Elevated Temperature on Salmonella typhi

Abstract Views: 333  |  PDF Views: 62

Authors

L. Krishnasamy
Department of Biotechnology, Hindustan College of arts & science, Kelambakkam-603103, Tamil Nadu, India

Abstract


The purpose of this study was to examine the effect of elevated temperature on S.typhi, which is water borne organism and cause the typhoid. It was incubated at various temperatures like 37°C, 42°C, and 47°C for overnight. Then the carbohydrate, lipid and the DNA content in each culture were measured. The results showed that an increase in temperature like 42°C and 47°C produced a marked decrease in the carbohydrates, lipid and DNA content when compared to the culture incubated at 37°C. Therefore slight increase in the temperature caused decrease in carbohydrate, lipid and the DNA content. When the organism is treated at a high temperature that can result in significant decrease in the micro & macromolecules, which ultimately leads to the death of the organism.

Keywords


Samonella typhi, Micro and Macromolecules, Lipids and Carbohydrates

References