Indian Journal of Innovations and Developments

Open Access Open Access  Restricted Access Subscription Access

Analysis of Phytochemical Components and Anti-microbial Activity of the Toxic Plant -Thevetia peruviana


Affiliations
1 Department of Biotechnology, Hindustan College of Arts and Science, padur, Kelambakkam-603103, Tamil Nadu, India
 

Medicinal plants are widely distributed in the country. Among these, the toxic plant Thevetia peruviana also comes under the medicinal plants category and it has many medicinal uses. The leaves of Thevetia peruviana were extracted with ethanol and then it was purified. Proteins and phenols were confirmed by performing TLC (Thin layer chromatography). Glycosides were confirmed by Borntrager's test. The purified sample was used to analyze the phytochemical compounds by GC/MS (Gas chromatography/Mass spectroscopy). The amount of glycosides present in the leaf extract was quantified using Spectrophotometer. Antimicrobial was tested against Escherichia coli, Salmonela typhi, Staphylococcus, Vibrio cholera and Shigella. The result showed the presence of proteins, phenols and glycosides in the leaf extract. Eleven phytochemical components were obtained GC/MS.

Keywords

Phytochemistry, Medicinal Plants, thevetia Peruviana, Toxic-plant, Chromatography
User
Notifications

  • Ban JO, Hwang IG, Kim TM, Hwang BY, Lee US, Jeong HS, Yoon YW, Kimz DJ and Hong JT (2007) Antiproliferate and pro-apoptotic effects of 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyranone through inactivation of NF-kappaB in human colon cancer cells. Cancer letters, 225, 41-52.

  • Eddleston Michael, Sheriff M H Rezvi and Hawton Keith (1998) Deliberate self-harm in Sri Lanka: an overlooked tragedy in the developing world. 317(7151), 133–135.

  • Omolara O (2007) Extractability of Thevetia peruviana glycosides with alcoholic mixture. Chemistry dept, University of llorin p.m.b 1515. AfricAN Journal of Biotechnology, 6(18), 2166-2170.

  • Shaw D and Pearn J (1979) Oleander poisoning. Med J Aust., 2(5), 267–269.

  • Thilagavathi R, Helen P. Kavitha and Venkatraman BR (2010) Isolation, Characterization and Anti-Inflammatory Property of Thevetia PeruvianaDepartment of Chemistry. Journal of science, 124, 477-484.

  • Usman (2009) The potential of yellow oleander in African Agriculture and Industrial development: a case study of Nigeria. Journal of Ethnopharmacology, 78, 139-143.


Abstract Views: 505

PDF Views: 111




  • Analysis of Phytochemical Components and Anti-microbial Activity of the Toxic Plant -Thevetia peruviana

Abstract Views: 505  |  PDF Views: 111

Authors

K. Mathuravalli
Department of Biotechnology, Hindustan College of Arts and Science, padur, Kelambakkam-603103, Tamil Nadu, India
R. Eswara Lakshmi
Department of Biotechnology, Hindustan College of Arts and Science, padur, Kelambakkam-603103, Tamil Nadu, India

Abstract


Medicinal plants are widely distributed in the country. Among these, the toxic plant Thevetia peruviana also comes under the medicinal plants category and it has many medicinal uses. The leaves of Thevetia peruviana were extracted with ethanol and then it was purified. Proteins and phenols were confirmed by performing TLC (Thin layer chromatography). Glycosides were confirmed by Borntrager's test. The purified sample was used to analyze the phytochemical compounds by GC/MS (Gas chromatography/Mass spectroscopy). The amount of glycosides present in the leaf extract was quantified using Spectrophotometer. Antimicrobial was tested against Escherichia coli, Salmonela typhi, Staphylococcus, Vibrio cholera and Shigella. The result showed the presence of proteins, phenols and glycosides in the leaf extract. Eleven phytochemical components were obtained GC/MS.

Keywords


Phytochemistry, Medicinal Plants, thevetia Peruviana, Toxic-plant, Chromatography

References