International Journal of Biotechnology and Bioengineering Research

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Studies of Antimicrobial Effects of Citrus Fruits along with their Peels


Affiliations
1 Department of Biotechnology, Institute of Biomedical Education & Research, Mangalayatan University, Aligarh (U.P.), 202145, India
2 Department of Biotechnology, Lovely Professional University, Phagwara-144402, Punjab, India
     

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Current study targeted the extraction and assay of antimicrobial metabolites from the peels. Peels were dried and antimicrobial metabolites were extracted from them by soxhlet extraction procedure. Extraction was done by solvents like cold water, hot water, methanol, ethanol, ethyl acetate and acetone. Extracts were subjected to antibacterial and antifungal susceptibility assay by agar well diffusion method. All the extracts of Lemon (Citrus lemon) were found to be effective against the used bacterial pathogens except hexane extracts. Methanol and acetone extract showed maximum zone of inhibition of 18 mm. Only methanol extract was effective against fungal pathogens showing a zone of inhibition of 18 mm. In case of orange (Citrus sinensis) hexane extract was found to be most effective against bacterial pathogens giving a zone of 13 mm. Only the cold water extract of orange was effective against fungal pathogens used in the study. Acetone extract of Mosambi (Citrus limetta) was most effective giving a zone of 20 mm against bacterial pathogens. Only cold water and ethyl acetate extracts of mosambi were effective against fungal pathogens giving a zone of inhibition of 17mm and 15mm respectively.

Keywords

Citrus Fruits, Antimicrobial Properties, Agar Well Diffusion Method, Soxhlet Extraction
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Abstract Views: 1269

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  • Studies of Antimicrobial Effects of Citrus Fruits along with their Peels

Abstract Views: 1269  |  PDF Views: 18

Authors

Dwivedi Shubham
Department of Biotechnology, Institute of Biomedical Education & Research, Mangalayatan University, Aligarh (U.P.), 202145, India
Bajpai Basant
Department of Biotechnology, Lovely Professional University, Phagwara-144402, Punjab, India
Pandey Kaushlendra Dutt Ram
Department of Biotechnology, Lovely Professional University, Phagwara-144402, Punjab, India

Abstract


Current study targeted the extraction and assay of antimicrobial metabolites from the peels. Peels were dried and antimicrobial metabolites were extracted from them by soxhlet extraction procedure. Extraction was done by solvents like cold water, hot water, methanol, ethanol, ethyl acetate and acetone. Extracts were subjected to antibacterial and antifungal susceptibility assay by agar well diffusion method. All the extracts of Lemon (Citrus lemon) were found to be effective against the used bacterial pathogens except hexane extracts. Methanol and acetone extract showed maximum zone of inhibition of 18 mm. Only methanol extract was effective against fungal pathogens showing a zone of inhibition of 18 mm. In case of orange (Citrus sinensis) hexane extract was found to be most effective against bacterial pathogens giving a zone of 13 mm. Only the cold water extract of orange was effective against fungal pathogens used in the study. Acetone extract of Mosambi (Citrus limetta) was most effective giving a zone of 20 mm against bacterial pathogens. Only cold water and ethyl acetate extracts of mosambi were effective against fungal pathogens giving a zone of inhibition of 17mm and 15mm respectively.

Keywords


Citrus Fruits, Antimicrobial Properties, Agar Well Diffusion Method, Soxhlet Extraction

References