International Journal of Advanced Biotechnology Research

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Estimation of Various Biochemical Parameters of Single Cell Protein Produced on Different Fruit Wastes Medium Using Saccharomyces cerevisiae and Candida Tropicalis


Affiliations
1 Department of Microbiology, Jawaharlal Nehru College, Shyamla hills, Bhopal (M.P.), India
     

   Subscribe/Renew Journal


The disposal of fruit wastes is a serious problem throughout the world. In the present investigation, different fruit wastes medium were prepared by mixing fruit wastes and distilled water in the ratio of 1:1. The fruit wastes medium were inoculated with two strains of yeast i.e. Saccharomyces cerevisiae and Candida tropicalis respectively. The single cell protein produced on these different fruit wastes medium were taken for analysis of different biochemical parameters like, analysis of moisture content, protein content, total carbohydrate content, and lipid content. The maximum moisture content was obtained on the SCP produced on orange fruit waste medium with C. tropicalis i.e. 15.2% and with S. cerevesiae i.e. 12.5% on banana fruit waste medium. The maximum protein content was obtained on the SCP produced on banana fruit wastes medium with S. cerevisiae i.e. 39.7% and with C. tropicalis i.e. 37.5% on pineapple fruit waste medium. The maximum total carbohydrate content was obtained on the SCP produced on orange fruit waste medium with S. cerevisiae i.e. 10.1% and with C. tropicalis i.e. 10.2% with mango fruit waste medium. The maximum lipid content was obtained with SCP produced on orange fruit waste medium i.e. 0.007% with S. cerevisiae and with C. tropicalis i.e. 0.006% on pomegranate fruit waste medium.

Keywords

Biochemical Parameters, Saccharomyces cerevisiae, Candida tropicalis, Fruit Wastes
Subscription Login to verify subscription
User
Notifications
Font Size


  • Argyro, B., Costas, P., Athanasios, A.K. 2006, Production of Food Grade Yeasts. Food Technol. Biotechnol. 44, 407-415

  • Nigam, N.M. 2000, Cultivation of Candida langeronii in sugarcane bagasse hemi cellulose hydrolysate for the production of single cell protein. W.J.Microbiol. and biotechnol. 16, 367-372.

  • Tipparat.,. H., Kittikun, A.H. 1985, Optimization of single cell protein production from cassava starch using Schwanniomyces castellii. W.J. Microbiol. & Biotechnol. 11, 607-609.

  • Abou Hamed, S.A.A. 1993, Bioconversion of wheat straw by yeast into single cell protein Egypt. J. Microbiol. 28 (1), 1-9.

  • Saquido, P.M.A. Cayabyab, V.A., Vyenco, R.R. 1981, Bioconversion of banana waste into single cell protein. J. Applied Microbiol. & Biotechnol. 5 (3), 321-326

  • Zhao, G., Zhang, W., Zhang, G. 2010, Production of single cell protein using waste capsicum powder produced during capsicum extraction. Lett Appl. Microbiol. 50, 187-91

  • Smith, M.E., Bull, A.T.1976, Studies of the utilization of coconut water waste for the production of food yeast Saccharomyces fragilis. J. Applied Bacteriol. 41 (1):81-95.

  • Karla, K.L., Grewal, H.S. and Kehlon , S.S. 1989, Bioconversion of kinnowmandarin waste in to single cell protein. J. Appl. Microbial Biotechnol. 5(3), 153-157

  • Rashad, M.M., Moharib, S.A.., Jwanny, E.W. 1990, Yeast conversion of mango waste or methanol to SCP and other metabolities. Biol. Waste. 32 (4), 277

  • Prosser, J.I., Tough, A.J. 1991, Growth mechanism and growth kinetics of filamentous microorganism. Critical Review in Biotechnol. 10(4), 253- 274.

  • Azin,M and N.Moazami.1989, Production of SCP from sugarcane bagasse. Second Congress of Forest and Technology. Faculty of Engineering. Tehran University, December 19-21.

  • Bellamy, W.D. 1975, Conversion of insoluble agricultural wastes to SCP by thermophilic microorganisms. In single cell protein II. S.R. Tannenbaum and D.I.C. Wang (Editors). MIT Press, Cambridge, Mass.

  • Dimmling, W. and Seipenbusch, R. 1978. Raw material for the production of SCP. Precess Biochem. 13 (3) 9-15.34

  • Enwefa, Chijioke. 1991, Biomass production from banana skins. Applied Microbiology and Biotechnology: 36(2):283-284 .

  • Kamel,Basil S. 1979, Dates as a potential substrate for single cell protein production. Enzyme and Microbial Technology: 1(3): 180-182

  • Khan,Y., Dahot,D., and Y. Khan 1992, Single cell protein production by Penicillium javanicum from pre-treated rice husk. J. Islam. Acad. Sci: 5: 39-43.

  • Mahat, M.S. and I.C. MacRae.1992, Rhizopus oligosporus grown on natural rubber waste serum for the production of single cell protein: a preliminary study. World J. of micro and Biotech: 8(1):63-64.

  • Moharib Sorial A.2003, Utilization of guava peel waste as substrate for protein and protease production by Saccharomyces cerevisiae. Advances in food sciences: 25(2):49-55.

  • Scrimshaw, NS.1975, Single cell protein for human consumption- an overview. In single cell protein II S.R. Tannenbaum and D.I.C Wang (Editors), MIT Press Cambridge, Mass.

  • Yousufi, Mahmood Khan; Khan, Shaukat Saeed and Jha, Krishna. 2003, The Effect of moisture content on the production of single cell protein using Rhizopus oligosporus and Aspergillus oryzae grown on okara – wheat grit Combinations. Indian J. Applied and Pure Bio. 18(1) 81-84.

  • Yousufi, Mahmood Khan. 2012, Study of Inoculum size, Incubation temperature and Nucleic Acid Concentration on the Single Cell Protein produced by using Soymilk residue (Okara). International Journal of Scientific & Engineering Research, 3(1): 1-4.

  • Yousufi. Mahmood Khan. 2012, Impact of pH on the single cell protein produced on okara wheat- grit substrates using Rhizopus oligosporus and Aspergillus oryzae. Journal of Environmental Science, Toxicology and Food Technology,1(2):32-35.

  • Yousufi, Mahmood Khan. 2012,Utilization of various fruit wastes as substrates for the production of Single Cell protein using Aspergillus oryzae and Rhizopus oligosporus. International Journal of Advanced Scientific and Technical research. 2(5): 92-95.

  • Yousufi, Mahmood Khan. 2012, To determine protein content of single cell protein produced by various combinations of fruit wastes and two standard food fungi. International Journal of Advanced Biotechnology and Research. 3(1):533-536.

  • AOAC.1984, Official methods of Analysis of the association of official agricultural chemists, 14th edn. Minnesote, USA.

  • Herbert, D., Phillips, P. J. and Strange, R. E. 1971, Chemical analysis of microbial cells. In: Methods in Microbiology vol 58, eds, J.R Norris And D.W Ribbons. Academic Press, London and New York, 344 pp.

  • Pearson, D., Melon, H.K., and Ronald, S. 1976, Chemical analysis of Food, 8th edition. Churchill Livingstone. Pp. 5-63.

  • 28 Kates M. 1982, In: Techniques of Lipidology Edn. P.H. Publ. Holland Publ. Co. Amsterdam. p. 106.


Abstract Views: 1191

PDF Views: 5




  • Estimation of Various Biochemical Parameters of Single Cell Protein Produced on Different Fruit Wastes Medium Using Saccharomyces cerevisiae and Candida Tropicalis

Abstract Views: 1191  |  PDF Views: 5

Authors

Mahmood Khan Yousufi
Department of Microbiology, Jawaharlal Nehru College, Shyamla hills, Bhopal (M.P.), India

Abstract


The disposal of fruit wastes is a serious problem throughout the world. In the present investigation, different fruit wastes medium were prepared by mixing fruit wastes and distilled water in the ratio of 1:1. The fruit wastes medium were inoculated with two strains of yeast i.e. Saccharomyces cerevisiae and Candida tropicalis respectively. The single cell protein produced on these different fruit wastes medium were taken for analysis of different biochemical parameters like, analysis of moisture content, protein content, total carbohydrate content, and lipid content. The maximum moisture content was obtained on the SCP produced on orange fruit waste medium with C. tropicalis i.e. 15.2% and with S. cerevesiae i.e. 12.5% on banana fruit waste medium. The maximum protein content was obtained on the SCP produced on banana fruit wastes medium with S. cerevisiae i.e. 39.7% and with C. tropicalis i.e. 37.5% on pineapple fruit waste medium. The maximum total carbohydrate content was obtained on the SCP produced on orange fruit waste medium with S. cerevisiae i.e. 10.1% and with C. tropicalis i.e. 10.2% with mango fruit waste medium. The maximum lipid content was obtained with SCP produced on orange fruit waste medium i.e. 0.007% with S. cerevisiae and with C. tropicalis i.e. 0.006% on pomegranate fruit waste medium.

Keywords


Biochemical Parameters, Saccharomyces cerevisiae, Candida tropicalis, Fruit Wastes

References