Problems: Since 2010 World Anti-Doping Agency (WADA) put glycerol in their prohibited substances list considering it is masking ability. Exogenous glycerol elevates plasma glycerol concentrations up to a point where glycerol begins to appear significantly in urine. Smoking hubbly bubbly that contained glycerol as humectant can affect the urinary glycerol threshold especially when a 41.3% of the Jordanian universities students smoke hubbly bubbly. The aim of the study is to assess the normal urinary glycerol range among the Jordanian athletes and determine hubbly bubbly influence on this range.
Experimental approach: Urine samples were collected from Jordanian athlete&nonathlete volunteers. In addition to urine sample from 20 Adult healthy Jordanian athletes who smoked hubbly bubbly. All samples were analyzed by GC/MS.
Findings: Analysis showed that 80% of the normal urinary glycerol concentration was ≤20μg/ml.
Conclusion: Hubbly bubbly regime used in Jordan showed no effect on urinary glycerol concentration.