Research Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Anti-oxidant properties of some medicinal plant products by ABTS Radical Scavenging Assay


Affiliations
1 Department of Life Sciences, Bangalore University, Bangalore – 560056., India
     

   Subscribe/Renew Journal


Antioxidant properties of plant products Cumin, Cinnamon, Pepper, Clove, and Star anise were measured against Quercetin as control by ABTS radical scavenging assay. The absorbance is recorded at 734nm, and percent inhibition is calculated at different concentrations of the sample. Graph pad prism method is used to calculate the IC50 values. A comparative account is tabulated in the study for the afore-mentioned plant products. Among the tested samples, Cinnamon has exhibited the highest IC50 value being 142 in chloroform extracts and the lowest being 18.09 is of star anise in methanol extracts.

Keywords

ABTS, Quercetin, free radical, antioxidant, reactive oxygen species.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Auddy B, Arredondo F, Blasina F, Dajas F, Ferreira M, Lafon L, Mukherjee B, Seal T, Tripathi PC, (2003). ‘Screening of antioxidant activity of Three Indian medicinal plants traditionally used for the management of neurodegenerative diseases J. Ethnopharmacol. 84, 131-138.

  • Buritis M. And Bucar F.(1999). Antioxidant activity of Nigella sativa essential oil. Phytother. Res., 14: 323 – 328.

  • De Zwart LL, Meerman JHN, Commandeur JNM, Vermeulen NPE (1999) ‘Biomarkers of free radical damage applications in experimental animals and in humans’. Free Rad.Biol.Med. 26(1/2), 202-226.

  • Duh, Pin-Der, Gow-Chin Yen, Wen-Jye Yen, and Lee?Wen Chang. "Antioxidant Effects of Water Extracts from Barley (Hordeum vulgare L.) Prepared under Different Roasting Temperatures", Journal of Agricultural and Food Chemistry, 2001.

  • Halliwell B, Gutteridge JMC and Aruoma OI. (1987). The deoxyribose method: a simple test tube assay-determination of rate constants for reaction of hydroxyl radicals. Anal. Biochem., 165 : 215-219.

  • Hemant R. Jadhav and K.K Bhutani. (2002). Antioxidant properties of Indian Medicinal plants. Phytother. Res., 16: 771 – 773.

  • L Lavanya, V. Veeraraghavan, CN Prashantha, Renuka Srihari. " In Vitro and Insilico screening platform for the identification of aldose reductase inhibitors for antidiabetic lead compounds from ", Cold Spring Harbor Laboratory, 2020

  • M. Gupta, U.K. Mazumder ., P. Gomath .. "Evaluation of Antioxidant and Free Radical Scavenging Activities of Plumeria acuminata Leaves", Journal of Biological Sciences, 2007

  • Sagrario Martin Aragon, Belen Basabe, Juana M. Benedi and Angel M. Villar. (1998). Antioxidant activity of VacciniummyrtillusL. Phytother. Res., 12: S104 – S106.

  • Slater TF (1984) ‘Free radical mechanisms in tissue injury’. Biochem. J. 222,1-15.

  • Vani T, Rajini M, Sarkar S and Shishoo CJ (1997), ‘Antioxidant properties of the Ayurvedic formulation-Triphala and its constituents’, Int.J.Pharmac. 35(5),313-317.

  • Valentão, P., E. Fernandes, F. Carvalho, P. B. Andrade, R. M. Seabra, and M. L. Bastos. "Antioxidant Activity of Centaurium erythraea Infusion Evidenced by Its Superoxide Radical Scavenging and Xanthine Oxidase Inhibitory Activity", Journal of Agricultural and Food Chemistry, 2001


Abstract Views: 131

PDF Views: 0




  • Evaluation of Anti-oxidant properties of some medicinal plant products by ABTS Radical Scavenging Assay

Abstract Views: 131  |  PDF Views: 0

Authors

Kavya Kulkarni
Department of Life Sciences, Bangalore University, Bangalore – 560056., India
Govindaiah
Department of Life Sciences, Bangalore University, Bangalore – 560056., India

Abstract


Antioxidant properties of plant products Cumin, Cinnamon, Pepper, Clove, and Star anise were measured against Quercetin as control by ABTS radical scavenging assay. The absorbance is recorded at 734nm, and percent inhibition is calculated at different concentrations of the sample. Graph pad prism method is used to calculate the IC50 values. A comparative account is tabulated in the study for the afore-mentioned plant products. Among the tested samples, Cinnamon has exhibited the highest IC50 value being 142 in chloroform extracts and the lowest being 18.09 is of star anise in methanol extracts.

Keywords


ABTS, Quercetin, free radical, antioxidant, reactive oxygen species.

References