Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Preliminary Phytochemical Screening and In Vitro Antioxidant Properties of the Pleurotus ostreatus extract


Affiliations
1 Columbia Institute of Pharmacy, Tekari, Raipur-493111 (C.G.), India
     

   Subscribe/Renew Journal


The free radical amassed in the body due to oxidative stress, and plays the leading part in the inception of disease. Presently researchers are working on natural antioxidant, because it has minimum toxic effect compared to chemical substances. Pleurotus ostreatus extract were evaluated for phytochemical composition and to assess in vitro antioxidant activities. In the course of preliminary phytochemical analysis, the petroleum ether extract, ethyl acetate extracts and ethanol extracts of P. ostreatus was evaluated to determine the presence of secondary metabolites. Antioxidant activity of the ethanol extract of P. ostreatus was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), Super oxide free radical scavenging assay (SOD) and total polyphenol content. Preliminary phytochemical screening of ethanol extract revealed the presence of phenolic compounds, flavonoids, alkaloids and glycoside, while petroleum ether and ethyl acetate extracts were devoid from these phytoconstituent. The ethanol extract scavenged DPPH radical and superoxide radical with the IC50 being 69.30 μg/ml and 107.05 μg/ml respectively. The ethanol extract of P. ostreatus revealed 83.47 ± 1.53 mg/100 gm of total polyphenol content. The finding endorses that P. ostreatus acquire high antioxidant activities, and it may uses as natural antioxidant for the healing of various diseases.

Keywords

Pleurotus ostreatus, DPPH, Antioxidant, Phytochemical.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Sharma S, Pal A. Preliminary Phytochemical Screening and In Vitro Antioxidant Activities of Parkinsonia aculeata Linn. BioMed Research International. 2014; 2014: 1-8.

  • Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. 2010, 10(21): 1-8.

  • Kenwat R, Prasad P, Sahu RK, Roy A, Saraf S. Preliminary Phytochemical Screening and In Vitro Antioxidant Efficacy of Fruit Oil of Martynia annua. UK Journal of Pharmaceutical and Biosciences. 2014; 2(1), 16-22.

  • Kumar G, Karthik L, Rao KVB. Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae). Asian Pacific Journal of Tropical Medicine. 2013; 180-187.

  • Arvind K, Singh S, Mahour K, Vihan VS, Gururaj K. Phytochemical Analysis of Some Indigenous Plants Potent Against Ectoparasite. Asian J of Exp Biol Sci. 2011; 2: 506-509.A

  • Sahu RK, Singh H, Roy A. Antioxidative characteristics of ethanol and aqueous extracts of Curcuma amada rhizomes. Research J. Pharmacognosy and Phytochemistry. 2009: 1(01): 41-43.

  • Sharma U, Sahu RK, Roy A, Golwala DK. In vivo antidiabetic and antioxidant potential of Stephania hernandifolia in streptozotocin-induced-diabetic rats. Journal of Young Pharmacists. 2010: 2(3): 255-260.

  • Pasko P, Barton H, Zagrodzki P, Gorinstein S, Fo1ta M, Zachwieja Z. Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chemistry. 2009; 115: 994-998.

  • Chlopicka J, Pasko P, Gorinstein S, Jedryas A, Zagrodzki P. Total phenolic and total flavonoid content, antioxidant activity and sensory evaluation of pseudo cereal breads. LWT - Food Science and Technology. 2012; 46: 548-555.

  • Gupta AK, Ahirwar NK, Shinde N, Choudhary M, Rajput YS, Singh A. Phytochemical Screening and Antimicrobial Assessment of Leaves of Adhatoda vasica, Azadirachta indica and Datura stramonium. UK Journal of Pharmaceutical and Biosciences. 2013; 1(1): 42-47.


Abstract Views: 290

PDF Views: 2




  • Preliminary Phytochemical Screening and In Vitro Antioxidant Properties of the Pleurotus ostreatus extract

Abstract Views: 290  |  PDF Views: 2

Authors

Amit Roy
Columbia Institute of Pharmacy, Tekari, Raipur-493111 (C.G.), India
Ram Kumar Sahu
Columbia Institute of Pharmacy, Tekari, Raipur-493111 (C.G.), India
Pushpa Prasad
Columbia Institute of Pharmacy, Tekari, Raipur-493111 (C.G.), India

Abstract


The free radical amassed in the body due to oxidative stress, and plays the leading part in the inception of disease. Presently researchers are working on natural antioxidant, because it has minimum toxic effect compared to chemical substances. Pleurotus ostreatus extract were evaluated for phytochemical composition and to assess in vitro antioxidant activities. In the course of preliminary phytochemical analysis, the petroleum ether extract, ethyl acetate extracts and ethanol extracts of P. ostreatus was evaluated to determine the presence of secondary metabolites. Antioxidant activity of the ethanol extract of P. ostreatus was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), Super oxide free radical scavenging assay (SOD) and total polyphenol content. Preliminary phytochemical screening of ethanol extract revealed the presence of phenolic compounds, flavonoids, alkaloids and glycoside, while petroleum ether and ethyl acetate extracts were devoid from these phytoconstituent. The ethanol extract scavenged DPPH radical and superoxide radical with the IC50 being 69.30 μg/ml and 107.05 μg/ml respectively. The ethanol extract of P. ostreatus revealed 83.47 ± 1.53 mg/100 gm of total polyphenol content. The finding endorses that P. ostreatus acquire high antioxidant activities, and it may uses as natural antioxidant for the healing of various diseases.

Keywords


Pleurotus ostreatus, DPPH, Antioxidant, Phytochemical.

References