Research Journal of Pharmacology and Pharmacodynamics

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Evaluation of Wound Healing, Anti-Inflammatory and Antioxidant Activity of Rhizomes of Curcuma longa


Affiliations
1 J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, MIDC, Hingna Road, Nagpur-440016, Maharashtra, India
2 Kamla Nehru College of Pharmacy, Wardha Road, Buttibori, Nagpur, MS, India
3 Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440033, MS, India
     

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The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn. rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200 μg/ml to 1000 μg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of curcuminoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

Keywords

Antioxidant, Wound Healing, Anti-Inflammatory, Curcuma longa.
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  • Kailong Yuan, Qianfeng Weng, Hongying Zhang, Jianhui Xiong, Guowang Xu Application of capillary zone electrophoresis in the separation and determination of the curcuminoids in urine. Journal of Pharmaceutical and Biomedical Analysis 38 (2005) 133-138.

  • P.T. Ammon, M.A. Wahl, Planta Med. 57 (1991) 1-7.

  • K.K. Soudamini, R. Kuttan, J. Ethno Pharmacol. 27 (1989) 227-233.

  • Masuda, A. Jitoe, Phytochemistry 32 (1993) 1557-1560.

  • R. Motterlini, R. Foresti, R. Bassi, C.J. Gree, Free. Radic. Biol. Med. 15 (2000) 1303-1312.

  • Koide, T., Nose, M., Ogihara, Y., Yabu, Y. and Ohta, N. (2002) Biol. Pharm. Bull. 25, 131-133.

  • Nanji, A.A., Jokelainen, K., Tipoe, G.L., Rahemtulla, A., Thomas, P. and Dannenberg, A.J. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284, G321-327.

  • Frautschy, S.A., Hu, W., Kim, P., Miller, S.A., Chu, T., Harris-White, M.E. and Cole, G.M. (2001) Neurobiol. Aging 22, 993-1005.

  • Janssen, Th. Gole, Chromatographia 18 (1984) 546-549.

  • M. Sanagi, U.K. Ahmad, R.M. Smith, J. Chromatogr. Sci. 31 (1993) 20-25.

  • Hiserodt, T.G. Hartman, C.T. Ho, R.T. Rosen, J. Chromatogr. A 740 (1996) 51-3.

  • H. Tonnesen, J. karlsen, J. Chromatogr. A 259 (1983) 367-371.

  • H. Khurana, J. Liq. Chromatogr. 11 (1988) 2296-2304.

  • X.G. He, L.Z. Lin, L.Z. Lian, M. Lindenmaier, J. Chromatogr. A 818 (1998) 127-132.

  • Prasad D. Rao CM. Indian J Pharm Sci 1995; 33:845.

  • Chitra P. Sajithal GB. Chandra KG. Indian J Exp Biology 1998; 36:896.

  • Jaswanth A. Akilandeswari LV. Manimaran S. Rukmani Indian J Pharm Sci 2001; 63:41.

  • Rang HP. Text book of pharmacology, International Student edition, Churchill living stone 1995: 246.

  • Rekka E. Kourounakis PN. J Pharm Pharmacol 1991; 43:486.

  • Morton JJP. Melon HM. Archives Internationales de Pharmcodynamic et de Therapie 1972; 176:117.

  • Suguna L. Siva Kumar P. Chandrakaran G. Indian J Exp Biology 1996; 34:1208-1211.

  • Lee KH. J. Pharmaceutical Sciences 1968; 1238-1240.

  • Ehrlich EH. Hunt TK Ibid 1969; 170:103-206.

  • Dipasquale G. Meli A. J Pharm Pharmacolgy 1965; 17:379.

  • Weossner JFT. Arch Biochem 1961; 93:440.

  • Yeo JH. Lee KW. Kim HC. Lyuny K. Biology and Pharmaceutical Bulletin 2000; 23:1220-1223.

  • Rao GV. Shiv Kuamr HG Parathasa RG. Indian J Pharamcology 1996; 28:249-253.

  • Thomas CA. Rama Sharma GVS. Indian Drugs 1999;6:203.

  • Duke JA. Bogen Schutz-Godwain M. Ducellier JD. Handbook of Medicinal herbs CRC press, London 2002.

  • Thripathy KP. Ananda VijayaKumar PR. Rajasekaran A. Indian Drugs 2001; 38:426.

  • Tenni R. J Biochem 1988; 37:169-177.

  • Sunitha S. Nagaraj M. Varalakshmip Fitotherapia 2001; 72:516-523.


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  • Evaluation of Wound Healing, Anti-Inflammatory and Antioxidant Activity of Rhizomes of Curcuma longa

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Authors

Rita M. Charde
J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, MIDC, Hingna Road, Nagpur-440016, Maharashtra, India
Hemant J. Dhongade
J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, MIDC, Hingna Road, Nagpur-440016, Maharashtra, India
Manoj S. Charde
Kamla Nehru College of Pharmacy, Wardha Road, Buttibori, Nagpur, MS, India
Siddheshwar B. Joshi
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440033, MS, India

Abstract


The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn. rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200 μg/ml to 1000 μg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of curcuminoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.

Keywords


Antioxidant, Wound Healing, Anti-Inflammatory, Curcuma longa.

References