Research Journal of Pharmacology and Pharmacodynamics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Cardioprotective Activity of Amritarishta on Isoproterenol Induced Myocardial Infarction


Affiliations
1 Department of Pharmacognosy, Dr K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), India
     

   Subscribe/Renew Journal


The present study was designed to evaluate the cardio protective activity of Amritarishta-T, Amritarishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Amritarishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Amritarishta significantly prevented the ISO-induced adverse changes in the levels of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Amritarishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords

Myocardial Infarction, Isoproterenol, Amritarishta.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Bolli R. Myocardial ischemic metabolic disorder leading to cell death. Rev Post Cardiol 1994; 13:649-53.

  • Dhar ML, Dhar MM, Dhawan BN, Ray C. Screening of Indian plants for biological activity. J Exp Biol 1968; 6:232-47.

  • Hertog MGL, Feskens EJM, Hollam PCH, Katan MB, Kromhout D. Dietary antioxidant flavonoids and risk of coronary heart diseases. Lancet 1993; 342:1007-20.

  • The Ayurvedic Formulary of India, Part-I. 2000, 1st edition, The Controller of Publications, Delhi, 6.

  • Kumar S, Verma NS, Pande D and Srivastava PS. In vitro regeneration and screening of berberinein Tinospora cordifolia. Journal of Medicinal and Aromatic Plant Science 2000;22:61.

  • Biset NG and Nwaiwu J.Quaternary alkaloids of Tinospora species. Planta Medica 1983;48:275-9.

  • Maurya R, Wazir V, Tyagi A and Kapil RS. Cordifoliosides A and B, two new phenylpropene disaccharides from Tinospora cordifolia possessing immunostimulant activity. Natural Product Letter 1996;8:7-10.

  • Gangan VD, Pradhan P, Sipahimalani AT and Banerji A. Cordifoliosides A, B, C:Norditerpene furan glycosides from Tinospora cordifolia. Phytochemistry 1994; 37:781-6.

  • Dixit SN and Khosa RL. Chemical investigation of Tinospora cordifolia. Indian Journal of Applied Chemistry 1971;34:46-7.

  • Maurya R and Handa SS. Tinocordifolin, a sesquiterpene from Tinospora cirdifolia. Phytochemistry 1998;49:1343-6.

  • Kidwai AR, Salooja KC, Sharma VN, Siddiqui S. Chemical examination of Tinospora cordifolia. Journal of Science and Indian Research 1949; 8:115-8.

  • Stanely M, Prince P and Menon VP. Antioxidant action of Tinospora cordifolia ischolar_main extract in alloxan diabetic rats.Phytotherapy Research 2001; 15:213-8.

  • Mehrotra R, Katiyar CK and Gupta AP. Hepatoprotective compositions and composition for treatment of conditions related to hepatitis-B and E infection. US Patent 749296. 2000.

  • Prince PS and Menon VP. Antioxidant activity of Tinospora cordifolia ischolar_mains in experimental diabetes. Journal of Ethnopharmacology 1999; 65:277-81.

  • Ikram M, Khattak SG and Gilani SN. Antipyretic studieson some indigenous Pakistani medicinal plants. Journal of Ethnopharmacology 1987; 19:185-92.

  • Manjrekar PN, Jolly CI and Narayanan S. Comparative studies of immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000; 71:254-7.

  • Jagetia GC, Nayak V and Vidyasagar MS. Evaluation of the antineoplastic activity of guduchi (Tinospora cordifolia) in cultured HeLa cells. Cancer Letter 1998;127:71-82.

  • Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.

  • Alam M, Radhamani S, Ali U and Purushottam KK. Microbiological Screening of Dhataki flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.

  • Rona G, Chapel CI, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Arch Pathol 1959;76:443-55.

  • Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi (India): Jaypee Brothers Medical Publishers Limited; 2008. p. 137-8, 537.

  • Varley H. Practical Clinical Biochemistry. 4th ed. NY: William Heinemann; 1967.p. 161-2.

  • Lamprecht W, Stan F, Weisser H, Heinz F. Determination of creatine phosphate and adenosine triphosphate with creatine kinase. In: Methods of Enzymatic analysis. Ed. HU Vergmeyer. NY: Academic Press; 1974. p. 1776-8.

  • Mohun AF, Cook IGY. Simple methods for measuring serum levels of Glutamic oxaloacetic and Glutamic pyruvic transaminases in routine laboratories. J Clin Pathol 1957;10 (4):394-9.

  • Allain CC, Pool LS, Chan CS, Richmond W. Enzymatic determination of serum cholesterol. Clin Chem 1974;20:447-75.

  • Friedewald WT, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18 :499-502.

  • Muller PH, Schmulling RM, Liebich HM, Eggstein M. A fully enzymatic triglyceride determination. J Clin Chem 1977;15:457-64.

  • Ohkawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

  • Ellman GL. Tissue Sulphydril groups. Arch Biochem Biophys 1959;82:72-7.

  • Nirmala C, Puvanakrishnan R. Isoproterenol induced myocardial infarction in rats; functional and biochemical alterations. Med Sci Res 1994;22:575-7.

  • Haenen GR, Veerman M, Bast A. Reduction of beta adrenoceptor functions by oxidative stress in heart. Free Radic Biol Med 1990;9:279-88.

  • Tappia PS, Heta T, Dhalla NS. Role of oxidative stress in catecholamine induced changes in cardiac sarcolemmal Ca2+ transport. Arch Biochem Biophys 2001; 377:85-92.

  • Pederson TR. Low density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. Am J cardiol 2001; 87(5A):8B-12B.

  • Bolden WE, Pearson TA. Raising low levels of High density lipoprotein cholesterol is an important target of therapy. Am J cardiol 2000;85(5):645-50.


Abstract Views: 351

PDF Views: 0




  • Cardioprotective Activity of Amritarishta on Isoproterenol Induced Myocardial Infarction

Abstract Views: 351  |  PDF Views: 0

Authors

Preeti Tiwari
Department of Pharmacognosy, Dr K. N. Modi Institute of Pharmaceutical Education and Research, Modinagar (U. P.), India

Abstract


The present study was designed to evaluate the cardio protective activity of Amritarishta-T, Amritarishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Amritarishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Amritarishta significantly prevented the ISO-induced adverse changes in the levels of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Amritarishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.

Keywords


Myocardial Infarction, Isoproterenol, Amritarishta.

References