Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

A Novel Bacterial Culture Media: Fruit Waste Agar


Affiliations
1 Department of Microbiology, Ahinsa Institute of Pharmacy, Dhule Road, Dondaicha, 425408, India
     

   Subscribe/Renew Journal


The development of microbiology began in the 19th century with the invention of the culture medium. Bacterial culture was the first method to study human microbiota in 1860. Louis pasteur was the first to propagate bacteria on culture media. The culture media provides the essential nutrients such as carbohydrates, protein, vitamins and some growth factors for the proper development of bacteria. Nowadays, these growth medias are prepared by different expensive chemical ingredients in laboratories for research experiments, which ultimately makes our experiments expensive. Instead of using high-cost culture media, fruit waste material could prove to be a good alternate source for the production of low-cost media. The waste generated in household practices and kitchen includes vegetables and fruit waste. It is waste that almost every house generates every day. It can serve as a good source of nutrients and vitamins for in microorganisms. Hence these materials can be used to formulate solid media for the growth of bacteria. In the current study waste material like mango peels, banana peels, lemon peels and ground nut shell have been included to formulate the media. This material was collected from kitchen waste and vegetable market. Comparing the growth of organism (lactobacillus bulgaricus) standard commercial media and fruit waste with agar, it was found that the media prepared from fruit waste serves as a good and inexpensive source of nutrients for many bacteria. Thus, it can good be feather and used commercially for isolation and cultivation of various microorganisms.

Keywords

Culture media, Bacteria, Fruit waste, Nutrients, Lactobacillus bulgaricus.
Subscription Login to verify subscription
User
Notifications
Font Size


  • M. Bornet, j.c lagier et.al, bacterial culture through selective and non-selective conditions: the evolution of culture media in clinical mirobiology (2020): 24(c)
  • Pratibha jadhav, maunalini sonne, aratí kadam, suraj patil, kirti dahigaonkar, jaspal kaur oberoi (2017): 10(2).
  • Sayali Daptardar, Darshana kankoshe, Shubhangi Salunke, Aman Dubey and Ashwini Rathod. Kitchen waste agar: A novel media for fungal cultivation (2018): 6(2): 2320- 2882.
  • John Lindquist, 2006, General microbiology A lab manual. 4th edition, mcgraw Hill primes custom publishing)
  • Hi Media Laboratories, Mumbai, Technical Data
  • Sunil Kumar, Stephen R. Smith, Geoff Fowler, costas velis, S. Jyoti kumar, Shashi Arya, Rena, Rakesh kumar and Christopher cheeseman. Challenges and oppurtunities associated with waste managment in india (2017):4: 160764
  • Janet I, Issac O, Mary N and Ijeeoma. Utilization of food crop wastes for the formulation of laboratory media used for cultivating soil fungi (2014): 2(1): 010-014)
  • Saheed Ok, Jamal P. Karim MIA, Alam Z, Muyibi SA. Cellulolytic, fruits: A potential support for enzyme assisted protein production (2013); 13(5): 379-385
  • Dievanayaki M, Antony Iruthayaraj P, Alternative vegitable nutrient source for microbial growth. (2012): 2(5): 47-51.
  • Ayesha Fatema Sayed, Culture Media and culture methods (2022) DOI: 10.13140 |RG22 27 20441.60009)
  • Power DA Johnson JA. Bitco™ and BBLTM manual, 2nd ed. Sparks: Becton, dikinson and Company; 2009)
  • Prof. Chandrakant kakore. Phamaceutical microbiology book, Nirali prakashan, 1st ed. (2018), Page No- 2.17
  • Dr. Kuntal Das, A text book. Of pharmaceutical microbiology, Second edition. (February 2019), Nirali prakashan, page no. 1.33
  • https://microbenotes.com/autoclave/
  • Veeranjaneya Reddy Labaka, Young-jung Wee, weibing Ye and Mallikarjuna korivi, Natritional composition and Bioctive Compounds in three different parts of mango fruit (2021): 18,741
  • https://en.m.wikipedia.org/wiki/Mangifera_
  • https://en.wikipedia.org/wiki/Banana
  • Ahmed M. Aboul enein et.al. Identification of phenolic compounds from banana peels (Musa paradaia L.) As ntioxidant and antimicrobial agents (2016): 8(4):46-55)
  • https://en.wikipedia.org/wiki/Peanut
  • Graudnut shells as a potential as feed supplement for ruminants on pasteures: A review. India Journal of animal research (2022): (56):521-524
  • https://en.wikipedia.org/wiki/Lemon
  • https://www.nutritionvalue.org/lemon_Peel
  • Anna Czech, Ewa zarycka et.al. Mineral content of the Pulp and Peel of various citous Past Cultivos (2020): 193: 555-563)
  • htpps://Sciencing.com/chemical-composition-nutrient-agar14135.html
  • https://microbenotes.com/streak-plate-method-principle-methodssignificance-limitations/
  • https://biologyreader.com/isolation-of-bacteria.html
  • https://noteshippo.com/spread-plate-method-in-microbiologyprinciple-procedure-uses-and-limitations/
  • https://microbonline.com/autoclave-principle-procedure-typesand-uses

Abstract Views: 258

PDF Views: 0




  • A Novel Bacterial Culture Media: Fruit Waste Agar

Abstract Views: 258  |  PDF Views: 0

Authors

Dhiraj S. Girase
Department of Microbiology, Ahinsa Institute of Pharmacy, Dhule Road, Dondaicha, 425408, India
Rahulsing G. Girase
Department of Microbiology, Ahinsa Institute of Pharmacy, Dhule Road, Dondaicha, 425408, India
Prasad P. Girase
Department of Microbiology, Ahinsa Institute of Pharmacy, Dhule Road, Dondaicha, 425408, India
Neha R. Jaiswal
Department of Microbiology, Ahinsa Institute of Pharmacy, Dhule Road, Dondaicha, 425408, India

Abstract


The development of microbiology began in the 19th century with the invention of the culture medium. Bacterial culture was the first method to study human microbiota in 1860. Louis pasteur was the first to propagate bacteria on culture media. The culture media provides the essential nutrients such as carbohydrates, protein, vitamins and some growth factors for the proper development of bacteria. Nowadays, these growth medias are prepared by different expensive chemical ingredients in laboratories for research experiments, which ultimately makes our experiments expensive. Instead of using high-cost culture media, fruit waste material could prove to be a good alternate source for the production of low-cost media. The waste generated in household practices and kitchen includes vegetables and fruit waste. It is waste that almost every house generates every day. It can serve as a good source of nutrients and vitamins for in microorganisms. Hence these materials can be used to formulate solid media for the growth of bacteria. In the current study waste material like mango peels, banana peels, lemon peels and ground nut shell have been included to formulate the media. This material was collected from kitchen waste and vegetable market. Comparing the growth of organism (lactobacillus bulgaricus) standard commercial media and fruit waste with agar, it was found that the media prepared from fruit waste serves as a good and inexpensive source of nutrients for many bacteria. Thus, it can good be feather and used commercially for isolation and cultivation of various microorganisms.

Keywords


Culture media, Bacteria, Fruit waste, Nutrients, Lactobacillus bulgaricus.

References