Research Journal of Pharmacognosy and Phytochemistry

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Antioxidant Profiling and Antibacterial Activity of the Vaccinium sikkimense from the Lachen Valley of the Sikkim Himalayas


Affiliations
1 State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
     

   Subscribe/Renew Journal


Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC50 value was calculated.

Keywords

V. sikkimense, Antibacterial Activity, Antioxidant Profiling, DPPH.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Burdulis Deividas, Aarkinas Antanas, Jasutien Ina , Ien Elicija Stackevi, Nikolajevas Laurynas and Janulis Valdimaras. Comparative Study of Anthocyanin Composition, Antimicrobial and Antioxidant Activity in Bilberry (Vaccinium Myrtillus L.) and Blueberry (Vaccinium Corymbosum L.) Fruits, Acta Poloniae Pharmaceutica and Drug Research, Vol. 66 No. 4 pp. 399n408, 2009.

  • Cheel C., Theoduloz T., Rodriguez C., Caligari J., Shemada Hirschmann G. Free radical scavenging activity and phenolic content in achenes and thalamus from Fragaria chiloensis, F. Vesca and F. Ananassa CV, Food Chemistry 102:36-44, 2007.

  • Harborne J.B., Phytochemical methods: A guide to modern techniques on plant analysis, third edition. Kluwer Academic Publisher, United Kingdom,1998.

  • Hooker J.D., Clarke C. B. The Flora of British India. (Fl. Brit. India), 3(9): 451, 1882.

  • Jayaveera. K.N, Yoganandham Reddy. K, Govindarajula Yadav, Kumanan. R. Phytochemical Screenings, Antibacterial Activity And Physico Chemical Constants Of Ethanolic Extract Of Euphorbia Thymifolia Linn, International Journal of Pharmacy and Pharmaceutical Sciences, Vol 2, Issue 3,2010.

  • Kumar Gaurav, Karthik Loganathan and Rao Kokati Venkat Bhaskara. Antimicrobial Activity of Latex of Calotropis Gigantea against Pathogenic Microorganisms-An In Vitro Study, Pharmacologyonline, 155-163, 2010.

  • Marinova, G. and Batchvarov, V. Evaluation Of The Methods For Determination Of The Free Radical Scavenging Activity By DPPH, Bulgarian Journal of Agricultural Science, 17 (No 1), 11-24,2011.

  • Mustarichie Resmi, Zalinar Linar Udin, Muchtaridi Supriyatna. Identification And Antibacterial Activity of Methanol Extract of Luffa acutangula Roxb, Medical and Health Science Journal, MHSJ; Volume 12, pp.70-77, 2012.

  • Parekh Jigna, Chanda Sumitra V. In vitro Antimicrobial Activity and Phytochemical Analysis of Some Indian Medicinal Plants, Turk J Biol, 31 53-58,2007.

  • Purkayastha Sharmishtha, Narain Rittee, Dahiya Praveen. Evaluation of antimicrobial and phytochemical screening of Fennel, Juniper and Kalonji essential oils against multi drug resistant clinical isolates, Asian Pacific Journal of Tropical Biomedicine, S1625-S1629,2012.

  • Rawat, G. S. and Tambe, S. Sikkim Himalaya: Unique Features Of Biogeography and Ecology; Biodiversity of Sikkim, Govt of Sikkim, 2011.

  • Rohman, A., Riyanto, S., Yuniarti, N., Saputra, W. R., Utami, R. and Mulatsih, W. Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam); International Food Research Journal. 17: 97-106,2010.

  • Singh Paramjit and Sanjappa. M. Flowering Plants of Sikkim-An Analysis.

  • Soni Anjali and Sosa Sheetal. Phytochemical Analysis and Free Radical Scavenging Potential of Herbal and Medicinal Plant Extracts. Journal of Pharmacognosy and Phytochemistry. 2 (4): 22-29, 2013.

  • Suganya. S., Bharathidasan. R., Senthilkumar. G., Madhanraj. P. and Panneerselvam. A. Antibacterial activity of essential oil extracted from Coriandrum sativam (L.) and GC-MS analysis, Journal of Chemical and Pharmaceutical Research 4(3):1846-1850, 2012.


Abstract Views: 370

PDF Views: 2




  • Antioxidant Profiling and Antibacterial Activity of the Vaccinium sikkimense from the Lachen Valley of the Sikkim Himalayas

Abstract Views: 370  |  PDF Views: 2

Authors

Smrita Pradhan
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
Sushen Pradhan
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
K. B. Subba
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India
Bharat Basistha
State Council of Science and Technology, Bigyan Bhawan, Deorali, Gangtok East Sikkim, India

Abstract


Vaccinium sikkimense under the family of Ericaceae is a low bush blueberry commonly found in Northern area of Sikkim Himalayas. The berry, leaves and the twigs of the plant were collected shade dried and grounded. The solvent extraction of the dried powder of the leaves, berry and the twigs and the fresh berry was carried out in different solvents to get a semi solid extract which was used for further studies like antibacterial activity against E.coli, P.aeruginosa, Klebsiella pneumoniae and S.aureus by well diffusion method where percentage inhibition was calculated using the formula and the graph was plotted. studies of the extract was carried out by DPPH method using UV/Vis spectrophotometer and the percentage scavenging activity of the reading was calculated using the formula and IC50 value was calculated.

Keywords


V. sikkimense, Antibacterial Activity, Antioxidant Profiling, DPPH.

References