Research Journal of Engineering and Technology

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Role of Aluminium and Phosphorus on the Secretion and Behaviour of Acid Phosphatase in Callus Culture of Brassica juncea


Affiliations
1 Department of Biotechnology, Bharathidasan University College, Perambalur-621 212, India
2 Department of Biotechnology, St. Joseph College, Trichy –620 002, India
3 P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamilnadu,, India
     

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The role of aluminium and phosphorus on the secretion of acid phosphatase in the callus culture of Brassia juncea was studied. Callus culture derived from leaves of mustard (Brassica juncea) plants. Proliferated calluses were subjected to P-deficient as well as P-sufficient conditions in the presence and absence of aluminium. The 10 days old calluses were harvested and so the culture medium in which they were cultured, for analysis of acid phosphatase, protein and amino acid content.

Keywords

Tissue Culture, Brassica juncea, Callus.
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  • Role of Aluminium and Phosphorus on the Secretion and Behaviour of Acid Phosphatase in Callus Culture of Brassica juncea

Abstract Views: 224  |  PDF Views: 1

Authors

R. Malathi
Department of Biotechnology, Bharathidasan University College, Perambalur-621 212, India
G. Melchias
Department of Biotechnology, St. Joseph College, Trichy –620 002, India
S. Chandrasekar
Department of Biotechnology, Bharathidasan University College, Perambalur-621 212, India
A. Cholarajan
P.G. Department of Microbiology, Meenakshi Chandrasekaran College of Arts and Science, Pattukkottai – 614 626, Tamilnadu,, India

Abstract


The role of aluminium and phosphorus on the secretion of acid phosphatase in the callus culture of Brassia juncea was studied. Callus culture derived from leaves of mustard (Brassica juncea) plants. Proliferated calluses were subjected to P-deficient as well as P-sufficient conditions in the presence and absence of aluminium. The 10 days old calluses were harvested and so the culture medium in which they were cultured, for analysis of acid phosphatase, protein and amino acid content.

Keywords


Tissue Culture, Brassica juncea, Callus.