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Optimization of Industrially Important Enzymes Laccase and Peroxidase Production in the Fungus Thelephora


Affiliations
1 Department of Biotechnology, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N., India
2 Department of Biotechnology, Bharathiar University, Coimbatore-641 046, T.N., India
3 Department of Biochemistry, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N, India
 

White rot fungus, Thelephora, isolated by its high production levels of ligninolytic enzymes from stumps of a burnt tree, was examined for its optimum growth conditions such as pH and temperature as well as carbon sources (glucose, cellulose and lignin) and nitrogen sources (diammonium tartrate, yeast extract and urea). For the maximum growth of Thelephora the 6 day incubation period was required at pH 5.0 and 35°C with 3.0 g/L lignin as a carbon source and 0.88 g/L yeast extract as a nitrogen source. The lignin peroxidase (LiP) production was maximal on the 7th day at pH 4.5 and 40°C with 3.0 g/L lignin and 0.44 g/L diammonium tartrate. The manganese-dependent peroxidase (MnP) production was achieved on 7th day incubation at pH 4.5 and 40°C, with 2.5 g/L glucose and 0.22 g/L diammonium tartrate as carbon and nitrogen sources respectively. The highest amount of laccase was obtained on the fourth day of incubation at pH 7.0 and 35°C using 3.0 g/L cellulose and 0.44 g/L diammonium tartrate as carbon and nitrogen sources respectively.
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  • Optimization of Industrially Important Enzymes Laccase and Peroxidase Production in the Fungus Thelephora

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Authors

K. Selvam
Department of Biotechnology, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N., India
K. Swaminathan
Department of Biotechnology, Bharathiar University, Coimbatore-641 046, T.N., India
M. Manikandan
Department of Biotechnology, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N., India
P. Chinnaswamy
Department of Biochemistry, Dr. N. G. P. Arts and Science College, Coimbatore-641 035, T.N, India

Abstract


White rot fungus, Thelephora, isolated by its high production levels of ligninolytic enzymes from stumps of a burnt tree, was examined for its optimum growth conditions such as pH and temperature as well as carbon sources (glucose, cellulose and lignin) and nitrogen sources (diammonium tartrate, yeast extract and urea). For the maximum growth of Thelephora the 6 day incubation period was required at pH 5.0 and 35°C with 3.0 g/L lignin as a carbon source and 0.88 g/L yeast extract as a nitrogen source. The lignin peroxidase (LiP) production was maximal on the 7th day at pH 4.5 and 40°C with 3.0 g/L lignin and 0.44 g/L diammonium tartrate. The manganese-dependent peroxidase (MnP) production was achieved on 7th day incubation at pH 4.5 and 40°C, with 2.5 g/L glucose and 0.22 g/L diammonium tartrate as carbon and nitrogen sources respectively. The highest amount of laccase was obtained on the fourth day of incubation at pH 7.0 and 35°C using 3.0 g/L cellulose and 0.44 g/L diammonium tartrate as carbon and nitrogen sources respectively.