Evaluation of Anti-Nociceptive and Anti-Inflammatory Effects of Hydroalcoholic Extract of Leaves of Oak, Quercus brantii by Formalin Test and Carraggeenan Model in Rat

The aim of the present study was to evaluate the anti-nociceptive and the anti-inflammatory activity of hydroalcoholic extract of Quercus brantii leaves by formalin test and carrageenan model in rat. In this work, 80 male-Wistar rats weighting about 210 ± 20 g were divided into ten groups of eight. Five groups were used for formalin test, and five groups for carrageenan model. For evaluation of anti-nociceptive effects the formalin test was used. Anti-nociceptive effect was determined in two phases. The minutes 0-5 and 16-60 were considered as acute and chronic phases of pain in the formalin test, respectively. The animals of experimental groups 1, 2 and 3 were pre-treated with oral doses of the extract at 200, 400, 600 (mg/kg) doses, 60 min before administration of formalin. For antiinflammatory effects, the carrageenan-induced hind paw oedema model in rats was used and the animals of experimental groups 1, 2 and 3 were pre-treated with oral doses of the extract at 200, 400, 600 (mg/kg), 30 min before administration of carrageenan. The control group without receiving any drug and the sham group receiving an equal volume of distilled water. The paw volume was measured in the mercury from 0 to 2 h and 30 min after carrageenan injection. Data were statistically analysed by ANOVA and t-tests (p<0.05). The results showed that the highest concentration of the extract 600 (mg/kg) decreased pain and inflammation in formalin test and carrageenan model in comparison with the control group (P< 0.05). The present study demonstrated that probably the anti-inflammatory and anti-nociceptive effects of oak leaf hydroalcoholic extract is related to available flavonoids and tannins presence in this extract.