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Evaluation of Lead Genotoxicity by Micronuclei Assay in Occupationally Lead Exposed Population


Affiliations
1 Human Genetics Lab, Department of Zoology, University College of Science, Osmania University, Hyderabad-500 007, A. P., India
2 Defence Institute of Physiology and Allied Sciences, Hyderabad, A. P., India
 

Lead, a ubiquitous element in the environment as a result of mining and industrialization, is found as a contaminant in humans. Lead is one of the heavy metals increasingly used for the petrol additives, and for various industrial purposes such as manufacture of storage batteries, solder manufacture, printing, smelting, painting, etc. In this study the lead genotoxicity was studied by using one of the recommended biomarker to assess environmental lead exposure is micronucleus assay. The blood samples were collected from 50 workers and 40 aged matched controls (5mL of venous blood from each lead exposed individual in heparinized sterile bottles). The battery manufacturers showed a significant increase in the frequency of micronuclei in comparison to that people who were not exposed to any toxicants. The results of present investigation indicate the clastogenic nature of lead in in vivo human lymphocytes of occupationally exposed population.

Keywords

Lead, Battery Manufacturers, Micronuclei, Genotoxicity.
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  • Evaluation of Lead Genotoxicity by Micronuclei Assay in Occupationally Lead Exposed Population

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Authors

N. Chandrashekhar Reddy
Human Genetics Lab, Department of Zoology, University College of Science, Osmania University, Hyderabad-500 007, A. P., India
K. Rudrama Devi
Human Genetics Lab, Department of Zoology, University College of Science, Osmania University, Hyderabad-500 007, A. P., India
Anjana Viz
Defence Institute of Physiology and Allied Sciences, Hyderabad, A. P., India

Abstract


Lead, a ubiquitous element in the environment as a result of mining and industrialization, is found as a contaminant in humans. Lead is one of the heavy metals increasingly used for the petrol additives, and for various industrial purposes such as manufacture of storage batteries, solder manufacture, printing, smelting, painting, etc. In this study the lead genotoxicity was studied by using one of the recommended biomarker to assess environmental lead exposure is micronucleus assay. The blood samples were collected from 50 workers and 40 aged matched controls (5mL of venous blood from each lead exposed individual in heparinized sterile bottles). The battery manufacturers showed a significant increase in the frequency of micronuclei in comparison to that people who were not exposed to any toxicants. The results of present investigation indicate the clastogenic nature of lead in in vivo human lymphocytes of occupationally exposed population.

Keywords


Lead, Battery Manufacturers, Micronuclei, Genotoxicity.