Biotransformation of Yellow 4G and Orange 2R Textile Dyes by Acclimatized Aerobic Bacteria

Degradation of the textile dyes viz., Yellow 4G and Orange 2R was carried out using acclimatized soil bacterial isolates. The microbial flora from soil was acclimatized to the dyes and six promising organisms were isolated, which could decolorize 1000 μg/mL of dyes to more than 91.00 % in nutrient medium in less than 24 hours. The percent decolorization of the dyes was determined by spectrophotometry. The six isolates reduced COD more than 75%. The degradation products formed after degradation were analysed by GC-MS technique and it was found that these cultures together degraded Yellow 4G and Orange 2R to the products having molecular weights 149, 65, 60, 57, 43, 41 and 271, 159, 145, 107, 102, 91, 81, 61, 55, 43 and 41 respectively.