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In Vitro Molecular Assessment of Cyanobacteria for Salt Tolerance


Affiliations
1 Dett. of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022,Distt. Rayagada, Orissa, India
 

Blue green algae (Cyanobacteria) constitute the largest, most diverse and most widely distributed group of photosynthetic prokaryotes. These are known to be primary colonizers of inhospitable ecosystems in which they colonizeed almost all kinds of aquatic ecosystems. Salt tolerance is a good taxonomic character too because of their diverse morphological organization and different biochemical machineries. Four species of cyanobacteria, namely Oscillatoria tenue, Lyngbya estruarii, Scytonema major and Calothrix juliana were collected from the salt pans of Huma and cultured in medium containing varying concentrations from0 to 25 ppt of NaCl for 20 days to asses the tolerance level of them. The growth and various macromolecular contents were determined along with the pigments on 20th day of culture. These organisms are able to tolerate the salt concentration of 5 ppt to 15 ppt.

Keywords

Cyanobacteria, Salt Tolerance, Molecular Assessment, Pigments.
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  • In Vitro Molecular Assessment of Cyanobacteria for Salt Tolerance

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Authors

Alekha Kumar Ghadai
Dett. of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022,Distt. Rayagada, Orissa, India
Diptikanta Acharya
Dett. of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022,Distt. Rayagada, Orissa, India
Sunil Kumar
Dett. of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022,Distt. Rayagada, Orissa, India
Sailabala Padhi
Dett. of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022,Distt. Rayagada, Orissa, India

Abstract


Blue green algae (Cyanobacteria) constitute the largest, most diverse and most widely distributed group of photosynthetic prokaryotes. These are known to be primary colonizers of inhospitable ecosystems in which they colonizeed almost all kinds of aquatic ecosystems. Salt tolerance is a good taxonomic character too because of their diverse morphological organization and different biochemical machineries. Four species of cyanobacteria, namely Oscillatoria tenue, Lyngbya estruarii, Scytonema major and Calothrix juliana were collected from the salt pans of Huma and cultured in medium containing varying concentrations from0 to 25 ppt of NaCl for 20 days to asses the tolerance level of them. The growth and various macromolecular contents were determined along with the pigments on 20th day of culture. These organisms are able to tolerate the salt concentration of 5 ppt to 15 ppt.

Keywords


Cyanobacteria, Salt Tolerance, Molecular Assessment, Pigments.