Estimation of Nisoldipine by RP-HPLC in Oral Solid Dosage Form

A reverse phase high performance liquid chromatographic method has been developed for the estimation of nisoldipine in tablet formulation. The separation was achieved by Phenomenex Gemini C₁₈ column (250×4.60mm, particle size 5ì) using water, acetonitrile and methanol (40:40:20) as mobile phase, at a flow rate of 1.5mL/min. Detection was carried out at 235 nm. Retention time of NIS was found to be 5.3min. The method has been validated for linearity, accuracy and precision. Linearity observed was in the range of 80-120μg/mL. The developed method was found to be accurate, precise, selective and rapid for estimation of nisoldipine in tablet dosage form.