A Conventional Synthesis and Characterization of some Novel Pyrazoles as Cytotoxic Agents

Present work deals with the synthesis, characterization and cytotoxic screening of novel substituted pyrazole derivatives. Different acetophenones (I) with various substituted aromatic aldehydes (II) were condensed into corresponding chalcones (1_a-e), which upon bromination gave chalcone dibromides (2_a-e). The treatment of 2_a-e with hydrazine hydrate, phenylhydrazine and 2,4-dinitrophenylhydrazine in the presence of TEA (triethanolamine) afforded different di and tri-phenyl substituted novel pyrazoles (3_a-d and 4_a-h). All the newly synthesized compounds were characterized by UV, FTIR, ¹H NMR, mass spectral analysis and elemental analysis. All the compounds were screened for anticancer activity against A549 cell line by SRB assay. Compounds 3_d, 4_b, 4_d, 4_f and 4_h showed promising cytotoxic potential.