Journal of Pharmaceutical Research

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A RP-HPLC method is developed for simultaneous estimation of paracetamol and ondansetron in bulk and oral suspension. The mobile phase used was a combination of potassium dihydrogen orthophosphate buffer: acetonitrile (adjusted to pH 3.0 using orthophosphoric acid) 60:40 v/v. The reversed phase column used was Thermo Hypersil BDS C8 (250 × 4.6 mm i.d., 5μm) at ambient temperature. The detection of the combined dosage form was carried out at 258 nm and a flow rate employed was 1.2 ml/min. The retention time for paracetamol and ondansetron was found to be 3.23min and 4.48min respectively. The method was validated in terms of linearity, range, accuracy, precision, limit of detection (LOD) and limit of quantitation (LOQ). The linearity for paracetamol and ondansetron was found in the concentration range of 31.25- 187.5 μg/ml and 0.5 - 3.0 μg/ml respectively. The percentage recoveries of paracetamol and ondansetron were found to be 100.29 and 100.39 respectively. The percentage relative standard deviation (% RSD) of paracetamol and ondansetron for intraday precision were found to be 0.165 and 0.169 respectively. The LOD and LOQ values for paracetamol and ondansetron were calculated. Thus the proposed method was successfully applied for simultaneous estimation of paracetamol and ondansetron for routine analysis.

Keywords

Paracetamol, Ondansetron and RP-HPLC.
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