Journal of Pharmaceutical Research

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A High Performance Thin Layer Chromatographic Determination of Clopidogrel Bisulphate in Tablets


Affiliations
1 Department of Pharmaceutical Chemistry, MES College of Pharmacy, Sonai, Tal - Newasa, Dist - Ahmednagar - 414 105 (M.S.), India
 

The present work describes validated Stability indicating High Performance Thin Layer Chromatographic (HPTLC) method for estimation of clopidogrel bisulphate in tablet. The method employed, TLC aluminum plates precoated with silica gel 60F-254 as a stationary phase and carbon tetrachloride:ethyl acetate:ammonia (5:0.3:0.2, v/v/v) as mobile phase. The wavelength selected for analysis was 230nm. Linerity was observed in concentration of 300-1500 ng. The percentage of labeled claim of clopidogrel was found 99.17%. The recovery was 99.20±0.04 as per peak area. The proposed method was evaluated statistically in terms of accuracy, precision, specificity and reproducibility and can be used for routine analysis of Clopidogrel bisulphate in tablets.

Keywords

Clopidogrel Bisulphate, HPTLC.
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  • A High Performance Thin Layer Chromatographic Determination of Clopidogrel Bisulphate in Tablets

Abstract Views: 331  |  PDF Views: 84

Authors

B. M. Narwate
Department of Pharmaceutical Chemistry, MES College of Pharmacy, Sonai, Tal - Newasa, Dist - Ahmednagar - 414 105 (M.S.), India
P. J. Ghule
Department of Pharmaceutical Chemistry, MES College of Pharmacy, Sonai, Tal - Newasa, Dist - Ahmednagar - 414 105 (M.S.), India
P. B. Mohite
Department of Pharmaceutical Chemistry, MES College of Pharmacy, Sonai, Tal - Newasa, Dist - Ahmednagar - 414 105 (M.S.), India
R. B. Ugale
Department of Pharmaceutical Chemistry, MES College of Pharmacy, Sonai, Tal - Newasa, Dist - Ahmednagar - 414 105 (M.S.), India

Abstract


The present work describes validated Stability indicating High Performance Thin Layer Chromatographic (HPTLC) method for estimation of clopidogrel bisulphate in tablet. The method employed, TLC aluminum plates precoated with silica gel 60F-254 as a stationary phase and carbon tetrachloride:ethyl acetate:ammonia (5:0.3:0.2, v/v/v) as mobile phase. The wavelength selected for analysis was 230nm. Linerity was observed in concentration of 300-1500 ng. The percentage of labeled claim of clopidogrel was found 99.17%. The recovery was 99.20±0.04 as per peak area. The proposed method was evaluated statistically in terms of accuracy, precision, specificity and reproducibility and can be used for routine analysis of Clopidogrel bisulphate in tablets.

Keywords


Clopidogrel Bisulphate, HPTLC.