Journal of Pharmaceutical Research

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


A simple, sensitive, precise and rapid high performance thin layer chromatographic (HPTLC) method has been developed for the quantitative estimation of abacavir sulphate and lamivudine in tablet dosage form. Abacavir sulphate and lamivudine were chromatographed on silica Gel 60 GF-254 TLC plate. The solvent system selected was methanol:acetone:n-butyl acetate in the ratio of 1:1:2 (v/v/v) and scanning was carried out in the absorbance mode at 284 nm. The spots were found compact for abacavir sulphate with Rf value of 0.58 and for lamivudine with Rf value of 0.35. Linearity of abacavir sulphate and lamivudine were in the range of 240-1200 ng/spot and 120-600 ng/spot, respectively with acceptable value of correlation coefficient. The method was validated for accuracy, precision, robustness and recovery studies. The limit of detection and quantification for abacavir sulphate were obtained 0.691 and 2.093 ng/spot, respectively, while for lamivudine 1.114 and 3.376 ng/spot, respectively. The suitability of this HPTLC method for quantitative determination of abacavir sulphate and lamivudine was proved by validation in accordance with the ICH guidelines. The developed method can be successfully applied in the routine analysis of commercial pharmaceutical tablets.

Keywords

Abacavir Sulphate, Lamivudine, HPTLC, Method Development and Validation.
User
Notifications
Font Size