Journal of Pharmaceutical Research

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Purpose: The present work describes analytical method development and validation for the estimation of Hydrochlorothiazide in different combinations using UPLC. Methodology: The separation was achieved on column ACE C18 (2μ x 3mm x 50mm), using mobile phases method A, B, C (0.5% Ammonium Acetate buffer solution: Methanol (50:50v/v)), D, E (0.005M KH2PO4 Buffer: Methanol: ACN (40:52:8) final PH-3 with orthophosporic acid.) respectively. The retention time of Hydrochlorothiazide were found to be 0.59, 0.60, 0.58, 0.61, 0.69 mins for method A, B, C, D, E, respectively with combination. Finding: The method was validated as per ICH, for Accuracy, Precision, Linearity and Robustness, specisity, LOD and LOQ. Conclusion: The developed and validated method was used to estimated the Hydrochlorothiazide in its different combinations. In all combinations % of assay were same, this method was used to routine quality control analysis.

Keywords

Hydrochlorothiazide, UPLC Method.
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